天麻诱导骨髓间质干细胞分化为神经元样细胞的实验研究

目的：探讨天麻对体外定向诱导大鼠骨髓间质干细胞(MSC)分化为神经元样细胞的影响：方法：通过贴壁法分离大鼠MSC，体外扩增培养。中药天麻定向诱导分化为类神经元样细胞光镜下观察细胞形态，免疫细胞化学法检测神经细胞特异性抗原标志物神经原烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)、巢蛋白(Nestin)。结果：大鼠MSC可通过贴壁法成功分离并可在体外大量扩增。天麻诱导2h后大部分可分化为神经元样细胞，出现胞体和突起，免疫细胞化学染色NSE、Nestin呈阳性，GFAP阴性。结论：大鼠MSC可诱导分化为神经元样细胞。

Experimental Study on Effect of Gastrodia in Inducing the Differentiation of Mesenchymal Stem Cells into Neuron-like Cells

OBJECTIVE: To investigate the effect of gastrodia in extracorporeal oriented inducing the differentiation of mesenchymal stem cells into neuron-like cells. METHODS: Mesenchymal stem cells were separated from bone marrow of rats by wall sticking method, amplifying cultured in vitro, and differentiated into neuron-like cells by oriented induction with gastrodia. The morphology of cells was observed under light microscopy, neuro-specific enolase (NSE), nestin and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry. RESULTS: Rats mesenchymal stem cells could be separated and amplified in vitro. After being induced by gastrodia for 2 hrs, most of the cells would be differentiated into meuron-like cells, revealing cytodendrite. By immunochemical staining, cells showed positive of NSE, nestin, and negative of GFAP. CONCLUSION: Rats' mesenchymal stem cells could be induced to differentiate into neuron-like cells.