实验性中耳积液中耳粘膜中纤维粘连蛋白及层粘连蛋白的 …

探讨细胞外基质成分－纤维粘连蛋和层粘连蛋白在中耳积液时中耳粘膜增生中的作用。方法 在内窥镜引导下３０只豚鼠行双侧咽鼓管管内烧灼，造成实验性中耳积液动物模型以过氧化酶标记的链霉卵白素法行中耳粘膜中纤维粘连蛋白，层粘连蛋白的检测。

Expressions of fibronectin and laminin in the middle ear mucosa with experimental middle ear effusions: immunohistochemical study]

OBJECTIVE: To understand the role of two components of extracellular matrix: fibronectin (FN) and laminin (LN) in the proliferated mucosa of otitis media with effusions. METHODS: A model of otitis media with effusions was established by coagulating the orifices of the eustachian tube under an endoscope in 30 guinea pigs. The expressions of FN and LN in the proliferated mucosa from these ears were detected using Streptavidin peroxidase (SP) method. RESULTS: In 60 ears with the orifices of the eustachinan tube coagulated, 28 ears showed otitis media with effusions. The increased expressions of FN on the fibroblasts and LN on the basement membranes and neovasculations were noted in the ears with otitis media with effusions. CONCLUSION: FN and LN may involve in the proliferated response of middle ear mucosa with otitis media with effusions. It may play a certain role in the occurrence of middle ear mucosa proliferations and adhesions.