| 理化处理对大豆凝集素活性的影响 |
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| 引用本文： | 戴大章,陈妙月,叶均安,刘建新. 理化处理对大豆凝集素活性的影响[J]. 营养学报, 2004, 26(3): 223-226 |
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| 作者姓名： | 戴大章 陈妙月 叶均安 刘建新 |
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| 作者单位： | 1. 浙江大学动物科学学院,杭州,310029
2. 浙江中医学院,杭州,310053 |
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| 基金项目： | 农业部饲料安全专向项目(农财函[2001]18号) |
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| 摘 要： | 目的:探讨四种理化处理方法对大豆凝集素(SBA)活性的影响。 方法:分别用干热、湿热、酸碱和金属离子处理大豆植物蛋白中的SBA,用血凝法测定SBA的凝集活力,观察经这些理化处理后SBA活性的变化。 结果:120℃以下的干热处理不能使SBA的活力明显丧失;而95℃、30min,100℃、20min或105℃、10min的湿热处理,可使SBA的活力完全丧失。在pH 1.0~4.5或pH 9~13范围内提取大豆蛋白质,可使SBA的活力显著丧失。大豆蛋白抽提液中三价、四价金属离子和部分二价、一价金属离子也可显著降低SBA的活力。 结论:在不影响大豆蛋白质品质的条件下,干热处理不能除去大豆中的SBA,适宜的湿热、酸碱和金属离子处理能有效钝化大豆中SBA的活性。
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| 关 键 词： | 理化处理 大豆凝集素活性 |
| 文章编号： | 0512-7955(2004)03-0223-04 |
| 修稿时间： | 2003-10-28 |
EFFECT OF PHYSICO-CHEMICAL TREATMENTS ON ACTIVITY OF SOYBEAN AGGLUTINTIN |
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| DAI Da-zhang,CHEN Miao-yue,YE Jun-an,LIU Jian-xin. EFFECT OF PHYSICO-CHEMICAL TREATMENTS ON ACTIVITY OF SOYBEAN AGGLUTINTIN[J]. Acta Nutrimenta Sinica, 2004, 26(3): 223-226 |
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| Authors: | DAI Da-zhang CHEN Miao-yue YE Jun-an LIU Jian-xin |
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| Affiliation: | DAI Da-zhang,CHEN Miao-yue1,YE Jun-an,LIU Jian-xin |
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| Abstract: | Objective: To investigate the effect of four kinds of inactivation methods on the activity of soybean agglutinin (SBA). Methods: Dry-heat, humid-heat, acid-alkali and metal ion were employed to evaluate their effects on the activity of SBA, which were quantified by hemagglutination. Results: Dry-heat below 120℃ did not obviously decrease the activity of SBA, while humid-heat (95℃ for 30 min,100℃ for 20 min or 105℃ for 10 min) completely inactivated the SBA. The activity of SBA significantly decreased when soybean protein was extracted under pH 1.0-4.5 or pH 9-13. Quadrivalent and trivalent metal ions markedly inhibited the activity of SBA. Conclusion: SBA can be inactivated effectively by humid-heat, acid-alkali or metal ion. Further study is needed to clarify whether there are synergistic effects between these treatments. |
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| Keywords: | physico-chemical treatment soybean agglutinin activity |
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