黄连药材不同部位、不同生长年限和不同海拔高度红外光谱法整体分析与评价

目的研究不同部位、不同生长年限和不同海拔高度黄连药材红外光谱的"指纹特征"以及盐酸小檗碱在黄连样品中含量的整体变化规律.方法借助于傅里叶变换红外光谱技术快速、非分离检测黄连样品不同部位、不同生长年限和不同海拔高度的红外光谱.结果黄连根茎、叶柄和须根的红外光谱图均具有明显的"指纹特征",盐酸小檗碱的含量趋势为:根茎＞叶柄＞须根;随着生长年限的增加黄连叶柄中盐酸小檗碱的含量随之增加,在海拔1 200m种植时,黄连叶柄中盐酸小檗碱的含量低于海拔为1 300、1 400、1 500m高度的含量;海拔高度、生长年限对黄连根茎中盐酸小檗碱的含量无影响.结论红外光谱技术能够较准确的把握黄连药材的整体质量,为规范种植黄连药材提供了一种检测方法.

Analysis and assessment of Coptis chinensis for different parts, ages, and heights using Fourier transform infrared spectroscopy

OBJECTIVE: To study the infrared (IR) fingerprint spectra of Coptis chinensis for different parts, ages, and heights, and to analyze the integrate rules about the content of berberine component in Coptis chinensis for different parts, ages, and heights. METHOD: The Fourier transform infrared (FTIR) spectroscopy was applied to detect the infrared spectra of Coptis chinensis samples rapidly and non-separately. RESULTS: The IR spectra of the root, leafstalk, and fibre of Coptis chinesis have their unique features. The content of berberine component in Coptis chinensis was different for different parts and the sequence: root > leafstalk > fibre. When Coptis chinensis grows, the content of berberine component in leafstalk also increases. The content of berberine component in leafstalk which planting in 1 200 m was less than that in 1 300, 1 400, and 1 500 m. The ages and heights provide no obvious influences on the content of berberine in the root of Coptis chinensis. CONCLUSION: Using FTIR spectroscopy, the quality of Coptis chinensis can be controlled, which provides a useful method for the standardized planting of Coptis chinensis.