| 半夏类药材提取物抗HeLa细胞活性研究 |
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| 引用本文： | 李娟,陈科力,黄必胜,雷湘.半夏类药材提取物抗HeLa细胞活性研究[J].中国医院药学杂志,2010(2). |
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| 作者姓名： | 李娟 陈科力 黄必胜 雷湘 |
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| 作者单位： | 广西植物研究所;湖北中医学院湖北省和教育部共建中药资源和中药复方重点实验室; |
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| 基金项目： | 湖北省教育厅重点科研项目(编号:2003A001); 国家科技支撑计划专题子项目(编号:2006BAI06A10-2-2) |
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| 摘 要： | 目的:研究半夏类药材提取物体外抗肿瘤的活性。方法:用流式细胞术检测半夏类药材提取物诱导HeLa细胞凋亡作用,并分析掌叶半夏提取物对细胞生长周期和Caspase-3表达的影响。结果:半夏类药材的抗肿瘤活性主要集中在总有机酸提取物或醋酸乙酯提取部位;掌叶半夏和半夏的抗肿瘤活性作用较强,水半夏相对较弱;抗肿瘤作用与抑制S期细胞增殖和激活Caspase家族诱导细胞凋亡有很大的关系。结论:半夏、水半夏和掌叶半夏的根茎,都有抗肿瘤的活性,以掌叶半夏活性最强;诱导细胞凋亡是它们抗肿瘤的作用机制之一。
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| 关 键 词： | 半夏 抗肿瘤 FCM Caspase-3 |
Study on anti-tumor activities on HeLa of extracts from Pinellia ternata and its allied species |
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| LI Juan,CHEN Ke-li,HUANG Bi-sheng,LEI Xiang.Study on anti-tumor activities on HeLa of extracts from Pinellia ternata and its allied species[J].Chinese Journal of Hospital Pharmacy,2010(2). |
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| Authors: | LI Juan CHEN Ke-li HUANG Bi-sheng LEI Xiang |
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| Institution: | LI Juan1,2,CHEN Ke-li2,HUANG Bi-sheng2,LEI Xiang2(1.Guangxi Institute of Botany,Guangxi Guilin 541006,China,2 Hubei College of Traditional Chinese Medicine,Key Laboratory of TCM Resource , TCM Compound Co-constructed by Hubei province , Ministry of Education,Hubei Wuhan 430065,China) |
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| Abstract: | OBJECTIVE To compare anti-tumor activities of different extracts from the medicinal plants Pinellia ternata and its allied species in vitro.METHODS FCM assays were used to detect cell apoptosis.And cell cycle,Caspase-3 expression of better extracts were analyzed.RESULTS The main active components were the ethyl acetate extracts or the parts of total organic acid.Among them,Rhizoma Pinelliae and Rhizoma Pinellia Pedatisectae were activer than Rhizoma Typhonii Flagelliforme.A further experiment concerning ext... |
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| Keywords: | Pinellia ternata anti-tumor FCM Caspase-3 |
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