MN 9202抗角叉菜胶引起的大鼠血栓形成作用及其对血液流变学的影响

目的：观察新的二氢吡啶类钙通道阻滞剂２，６－二甲基－４－（３－硝基苯基）－１，４－二氢－３，５－吡啶二羧酸－３－甲酯－５－正戊酯（ＭＮ９２０２）对角叉菜胶引起的大鼠血栓形成的影响，探讨其抗血栓形成作用的血液流变学机制。方法：大鼠ｓｃ角叉菜胶５ｍｇ·ｋｇ－１复制血栓模型。给药后观察大鼠尾部血栓形成状况，进行血液流变学分析。结果：ＭＮ９２０２０．１、１、１０μｍｏｌ·ｋｇ－１能对抗角叉菜胶引起的大鼠血栓黑尾的形成（Ｐ＜０．０５，Ｐ＜０．０１），呈现出明显的剂量依赖关系（ｒ＝０．９９９５）；０．０１，０．１，１，１０μｍｏｌ·ｋｇ－１能降低ＲＢＣ聚集指数（Ｐ＜０．０５，Ｐ＜０．０１），增强ＲＢＣ变形性（Ｐ＜０．０５，Ｐ＜０．０１），但不改变ＲＢＣ电泳迁移率；大剂量的ＭＮ９２０２１，１０μｍｏｌ·ｋｇ－１能降低切变率为１５０·ｓ－１、５０·ｓ－１、１·ｓ－１时全血粘度。结论：ＭＮ９２０２具有对抗角叉菜胶引起的大鼠血栓形成作用。其作用可能与其改善血液流变性特征有关。

Effect of methyl pentyl 1,4 dihydro 2,6 dimethyl 4 (3 nitrophenyl) 3,5 pyridinedicarboxylate(MN 9202) on thrombosis and blood rheology in rats induced by carrageenan

AIM: To observe the effect of MN 9202 on thrombosis and blood rheology in rats induced by carrageenan. METHOD: SD rats of male were used in this experiment. The thrombus model was induced by a single sc dose of 1% carrageenan (05 ml·kg-1). Saline, vehicle or MN 9202 at dosage of 001, 01, 1, 10 μmol·kg-1 was given ip before and after injection of carrageenan. The effects of MN 9202 on the length of black tail and parameter of blood rheology were observed. RESULTS: MN 9202 at dosage of 01, 1, 10 μmol·kg-1 prevented thrombosis on tails induced by carrageenan (length of black tail: 28±15, 21±20, 17±12 vs 48±18 mm P<005, P<001, r=09995). MN 9202 enhanced the deformation of RBC (038±002, 037±002, 035±003, 035±002 vs 040±001 P<005, P<001), deduced the aggregation index of RBC (135±034, 106±007, 122±012, 141±022 vs 193±026 P<005, P<001), and deduced the whole blood vicosity at high dosage (1 and 10 μmol·kg-1), but did not affect the RBC electrophoretic mobility. CONCLUSION: These results indicate that MN9202 prevents thrombosis induced by carrageenan, in which the mechnism associates with its improving the characteristics of blood reheology.