肠内营养加黄芪对创伤机体CD4+/CD8+影响的研究

目的研究肠内营养加黄芪对创伤大鼠细胞免疫功能CD4+/CD8+的影响。方法 40只SD大鼠,随机分为4组,每组10只,即正常对照组(A),创伤对照组(B),创伤后肠内营养治疗组(C)和肠内营养加黄芪治疗组(D)。双侧股骨离断及胃管置入术建立创伤及肠内营养模型,早期(创伤后3～5h)给予肠内营养及肠内营养加黄芪连续治疗6d,取血测定免疫指标T细胞表型CD4+,CD8+含量。结果创伤后大鼠肠内营养及加黄芪治疗后,CD4+细胞、CD4+/CD8+值C,D组均高于B组(P<0.05),以D组明显(P<0.01)。结论创伤后大鼠细胞免疫功能下降,早期肠内营养及肠内营养加黄芪可改善其细胞免疫功能,对创伤机体具保护作用。

Effect of enteral nutrition and astragalus on cellular immune function CD_4~+/CD_8~+ of rats traumatized

Objective To study the effects of enteral nutrition and enteral nutrition add astragalas on cellular immune function CD4^＋/CD8^＋ of rats traumatized. Methods Forty SD rats were randomly divided into four groups （n=10）： group A, without trauma （normal control）, group B（traumatic control）, group C（enteral nutrition ） and group D （enteral nutrition add astragalus） with bilateral femur fractures . The latter two groups received early enteral nutrition and enteral nutrition add astragalus began at 3 - 5 hours following stomach intracaval catheterization after trauma for 6 days, blood were obtained from heart of rats. CD4^＋ cells and CD8^＋ cells were analysed by FACS. Results CD4^＋ cells counts and CD4^＋/CD8^＋ ratio were significantly decreased after trauma（P〈0.01）. In groups C and D, CD4^＋ cells counts and CD4^＋/CD8^＋ ratio were less decreased than group B （P〈0.05 in group C, P〈0.01 in group D）. Conclusion Bilateral femur fractures impair the cellular immune function of rats. The early enteral nutrition add astragalus improve host immune response to trauma.