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| 反义寡核苷酸对Daudi细胞α2,6唾液酸的下调作用研究 | |
| 引用本文： | 林绍强,尹小菁,李君武,李扬秋,丁彦青.反义寡核苷酸对Daudi细胞α2,6唾液酸的下调作用研究[J].肿瘤防治研究,2004,31(6):338-340. |
| 作者姓名： | 林绍强 尹小菁 李君武 李扬秋 丁彦青 |
| 作者单位： | 1. 510632,广州,暨南大学医学院微生物与免疫学教研室 2. 广州市第六人民医院内科 3. 暨南大学医学院血液病研究所 4. 第一军医大学肿瘤研究所 |
| 基金项目： | 国家重点基础研究发展计划(973计划) |
| 摘 要： | 目的 研究反义寡核苷酸对Burkitt’s淋巴瘤Daudi细胞α2 ,6 唾液酸转移酶 (ST6GalI)的活性和细胞表面α2 ,6 唾液酸水平的下调作用。方法 以修饰后的ST6GalI的反义寡核苷酸处理Daudi细胞 ,以RT PCR检测Daudi细胞ST6GalImRNA的表达 ,荧光定量法测定ST6GalI的活性及以流式细胞仪检测细胞表面α2 ,6 唾液酸的含量。结果 和对照组比较 ,以反义寡核苷酸处理的Daudi细胞ST6GalImRNA的表达下调 ,ST6GalI酶活性下降 ,并导致细胞表面α2 ,6 唾液酸水平降低。结论 反义寡核苷酸可有效降低Daudi细胞表面α2 ,6 唾液酸化水平 ,内源性减少α2 ,6 唾液酸对Daudi细胞CD2 2受体的屏蔽作用 |
| 关 键 词： | 反义寡核苷酸 唾液酸转移酶 Burkitt’s淋巴瘤 CD22 |
| 文章编号： | 1000-8578(2004)06-0338-03 |
| 收稿时间： | 2004-2-17 |
| 修稿时间： | 2004-4-14 |
A Study of Antisense-Oligonucleotides in Down-Regulation of α2,6-Sialylation of Daudi Cells |
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| LIN Shao qiang ,YIN Xiao jing ,LI Jun wu ,LI Yang qiu ,DING Yan qing .Dept. of Microbiology and Immunology of Medical College,Ji'nan University,Guangzhou ,China,.Dept. of Intern Medicine,the th People's Hospital of Guangzhou.A Study of Antisense-Oligonucleotides in Down-Regulation of α2,6-Sialylation of Daudi Cells[J].Cancer Research on Prevention and Treatment,2004,31(6):338-340. | |
| Authors: | LIN Shao qiang YIN Xiao jing LI Jun wu LI Yang qiu DING Yan qing Dept of Microbiology and Immunology of Medical College Ji'nan University Guangzhou China Dept of Intern Medicine the th People's Hospital of Guangzhou |
| Institution: | LIN Shao qiang 1,YIN Xiao jing 2,LI Jun wu 1,LI Yang qiu 3,DING Yan qing 41.Dept. of Microbiology and Immunology of Medical College,Ji'nan University,Guangzhou 510632,China,2.Dept. of Intern Medicine,the 6th People's Hospital of Guangzhou,3.Institute of Hematology of Medical College,Ji'nan University,4.Tumor Insitute of 1st Military Medical University |
| Abstract: | Objective To study the antisense oligonucleotides (antisense ODN) in down regulation of α2,6 sialyltransferase (ST6Gal I) activity and α2,6 sialylation of cell surface of Burkitt s lymphoma cell line Daudi.Methods Daudi cells were treated with modified antisense ODN in vitro, the ST6Gal I mRNA expression was examined by RT PCR, the ST6Gal I enzyme activity was measured with fluorometric sialyltransferase assay and the amount of α2,6 sialylation of cell surface was determined by FACS analysis.Resul... |
| Keywords: | CD22 |
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