Characterization and subcellular localization of the dystrophin-protein 71 (Dp71) from brain |
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Authors: | Daniel Jung Dominique Filliol Marie-Helene Metz-Boutigue and Alvaro Rendon |
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Institution: | INSERM U338, 5 rue Blaise Pascal, 67084, Strasbourg cedex, France |
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Abstract: | In the present study, monoclonal antibodies raised against the C-terminal domain of dystrophin were used to identify and characterize Dp71 from the central nervous system. It was observed that the expression of Dp71 gradually increases from the embryo stage until the adult. Subcellular distribution analysis indicates that Dp71 is mainly recovered in synaptic plasma membranes, microsomes and at a lesser extent in synaptic vesicles and mitochondria. The amino acid composition and N-terminal sequence of bovine brain Dp71 were determined. Moreover, we found that this protein is glycosylated. |
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Keywords: | Duchenne muscular dystrophy (DMD) central nervous system dystrophin dystrophin-protein 71 |
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