^51Cr测定肝硬化大鼠小肠转运时间及其意义

目的估价肝硬化大鼠的小肠转运时间,确定最有效的参数.方法同批在同样条件饲养的正常SD大鼠30只,分为3即即阿托品组、西沙比利组及正常对照组各10只.20只大鼠用于肝硬化模型制作,肝硬化模型采用CCl4皮下诱导,皮下注射50%CCl4-橄榄油溶液.小肠转运时间测定用含有2μCi51Cr的磷酸缓冲液1 ml经导管在十二指肠直接灌注,并采用下列参数评价(1)放射性在小肠中的分布;(2)最远端的放射性;(3)几何中心及几何中心比率.结果阿托品组,肝硬化组及正常大鼠组的放射性物质大多潴留在第一区域.阿托品组最为明显.西沙比利组在30′时放射性物质集中在第3区域,在60′时推进到第4区域.60′最远端的放射性物质推进较30′时明显.30′及60'时正常大鼠及西沙比利组大鼠的几何中心及几何中心比率明显高于肝硬化大鼠及阿托品组,西沙比利组大鼠30′时与正常组比较无差异,但60′时两者比较有显著差异,P＜0.05.结论所用3个参数所得的转运时间不完全一致,以远端放射性参数最差,最不敏感,几何中心及几何中心比率的相关性最好.肝硬化存在着小肠转运障碍.应用51Cr时30′完全能够满足对肝硬化小肠动力的研究.

Significance of 51Cr in Determination of Intestinal Transit of Cirrhotic Rats

Objective: To evaluate intestinal transit of cirrhotic rats and determine the most useful parameter of 51Cr.Methods: 30 normal SD rats in the same fed condition were divided into three groups: atropine,cisapride and normal control. Cirrhotic rats were induced by injection of 50% CCl_4 solution subcutaneously for 12 weeks. Intestinal transit was measured by 51Cr intradeodenum infusion and evaluated with following parameters: 1.distribution of radioactive substance in intestine. 2. the most distal edge of radioactive substance and 3. geometric center orgeometric ratio.Results: Most of radioactive substance was stayed in first area of intestine in atropine-treated, cirrhotic and normal rats. At 30 min radioactive substance were promoted to third area and at 60 min to fouth area in cisapride-treated group. Geometric center or geometric center ratio at 30 and 60 min in normal and cisapride-treated rats were higher than that of cirrhotic and atropine-treated rats. At 30 min there was no difference between cisapride-treated and normal rats but at 60 two group showed difference.Conclusion:Intestinal transit time by three parameters were not in accordance, with poorest of the most distal edge of radioactive substance and the best of geometric center or geometric center ratio. Intestinal transit was delayed in cirrhotic rats. 30min after 51Cr infusion was enough to study intestinal transit in cirrhotic rats.