肝细胞癌MSCT多期增强扫描与Survivin、VEGF及MVD表达关系的研究

目的:探讨肝细胞癌(HCC)的MSCT多期增强扫描与Survivin、VEGF表达及微血管生成之间的关系。方法:对36例病理证实为HCC的患者术前行MSCT平扫及增强扫描,术后标本进行免疫组化染色测定Survivin、VEGF以及MVD表达情况,将MSCT强化特征与免疫组化结果进行分析。结果:①36例HCC组织中Survivin表达阳性率为69.4%(25/36),VEGF表达阳性率72.2%(26/36)。26例VEGF表达阳性的HCC中,20例Survivin表达阳性,Survivin表达与VEGF表达呈显著正相关。②HCC的MSCT征象中肿瘤大小及瘤体中心有无坏死与Survivin、VEGF的表达无关(P>0.05)。有静脉癌栓组的Survivin表达明显高于无癌栓组(P<0.05)。边缘不清、有静脉癌栓组的VEGF表达明显高于边缘清楚、无静脉癌栓组(P<0.05)。MVD值表达与VEGF表达相同。结论:Survivin、VEGF及MVD在HCC呈高表达且三者密切相关,HCC的MSCT表现特征在一定程度上能反映Survivin、VEGF的表达以及肿瘤微血管生成情况,可以在一定程度上从分子水平判断HCC的侵袭、转移。

Correlative study between Survivin,VEGF,MVD and MSCT multi-phase scan in hepatocellular carcinoma

Objective： To investigate the correlation between multi phase multi-slice computed tomography（MSCT）imaging lea tures and the expression of Survivin, vascular endothelial growth factor （VEGF）, microvascular density （MVD） of HCC. Methods： Thirtysix HCC cases proved by histopathology were examined with multi-phase contrast-enhanced MSCT scanning. After operation,the specimens were used immunohistoehemical staining to detect the expression of Survivin, VEGF and MVD. Results： （1） Of 36 cases advanced hepatoellular carcinoma, the positive expression rate of survivin and VEGF were 69.4% and 72.2% respectively. In 36 cases, both Survivin expression and VEGF positive expression were detected in 20 speci- mens,and the expression of Survivin was positive correlation with VEGF. （2）The boundary of tumor being demarcated or blurred,portal vein tumor thrornbus was closely correlated to the expression of VEGF（ P〈0.05）. The portal vein tumor thrombus was correlated to the expression of Survivin（ P〈0.05）. The tumor center having necrosis or unnecrosis was not correlated to MVD and VEGF（ P 〉0.05）. There was no significant relationship between MSCT feature and the expression of Sur vivin and VEGF in tumor＇s diameter enhancement of AP or center necrosis（ P 〉0. 05）. Conclusion： The expression of Survivin, VEGF and MVD in HCC is significantly high and correlated. MSCT imaging features could reflect tumor angiogenesis in certain extent and infer the expression of Survivin and VEGF,predicting infiltration and metastasis of HCC from molecular level.