| 创伤后应激障碍大鼠海马神经元自噬增强 |
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| 引用本文： | 隋竹欣,刘昊,朱玉英,熊佩黎,纪荣静,王海涛. 创伤后应激障碍大鼠海马神经元自噬增强[J]. 神经解剖学杂志, 2013, 29(3): 325-328 |
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| 作者姓名： | 隋竹欣 刘昊 朱玉英 熊佩黎 纪荣静 王海涛 |
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| 作者单位： | 1. 河北联合大学基础医学院组织胚胎学系,唐山,063000
2. 河北联合大学附属医院神经内科,唐山,063000 |
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| 基金项目： | 国家自然科学基金，河北省自然基金项目，河北联合大学大学生创新性实验计划 |
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| 摘 要： | 目的:观察创伤后应激障碍(PTSD)模型大鼠海马神经元自噬的改变,探讨PTSD致海马体积异常的可能机制。方法:成年健康雄性SD大鼠20只,随机被分为对照组和模型组。采用改良的单一连续应激后给予不可逃避足底电击方法制备PTSD大鼠模型;采用透射电镜技术观察海马神经元超微结构,Western blot方法检测海马微管相关蛋白轻链3(microtube-associated protein 1 light chain 3,LC-3)和Beclin-1的表达水平。结果:模型组海马神经元自噬体增多;海马组织LC3-Ⅱ/LC3-Ⅰ比值和Beclin-1表达水平高于对照组(P<0.05)。结论:PTSD模型大鼠海马神经元存在明显的细胞自噬,可能与海马体积异常变化有关。
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| 关 键 词： | 创伤后应激障碍 海马 自噬 大鼠 |
Enhancement of autophagy in hippocampus of rat with post-traumatic stress disorder |
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| Abstract: | Objective: To observe the change of autophagy in hippocampal neurons of rat with post-straumatic stress disorder(PTSD),and to investigate the possible mechanism of hippocampal volume alteration in PTSD.Methods: Twenty adult male SD rats were randomly divided into control group and PTSD model group.The PTSD animal model was established by using the modified single-prolonged stress followed by a single inescapable electric foot shock(SPS & S).The ultrastructure of neurons in hiappocampus was observed under electron microscope.The expression of LC-3 and Beclin-1 protein in hippocampus was detected by Western blot.Results: Autophagosomes increased in hippocampal neurons of PTSD model rats.The ratio of LC3-Ⅱ to LC3-Ⅰand the expression of Beclin-1 protein were higher in model rats than in control rats(P<0.05).Conclusion: Atuophagy was enhanced in hippocampal neurons in rats with PTSD,which might be related to the abnormal change of hippocampal volume in PTSD. |
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| Keywords: | post-traumatic stress disorder hippocampus autophagy rat |
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