伤科止痛膏对急性软组织损伤大鼠损伤组织中一氧化氮及超氧化物歧化酶的影响

目的:观察伤科止痛膏对大鼠急性软组织损伤后损伤组织中一氧化氮(NO)和超氧化物歧化酶(SOD)影响以及局部软组织的形态学改变。方法:采用机械冲击挫伤的造模方法,建立大鼠急性软组织损伤模型,分别应用伤科止痛膏及扶他林乳膏涂布患处,并于治疗后第1,3,5天取材,测定大鼠损伤症侯指数、组织学及形态计量学指标,同时测定损伤组织中NO和SOD含量。结果:伤科止痛膏对急性软组织损伤动物模型有良好的恢复作用,给药组在损伤症侯指数、组织学恢复方面优于模型对照组,并能显著抑制损伤组织NO含量的升高(P0.01),增高损伤组织SOD活性(P0.01)。结论:伤科止痛膏治疗急性损伤的疗效机制之一与增高损伤组织SOD活性,降低局部软组织细胞释放的自由基NO含量有关。

Effect of Shangke Zhitong Plaster on Nitric Oxide and Superoxide Dismutase Level in Rat Model of Acute Soft Tissue Injury

Objective: To observe the effect of Shangke Zhitong Plaster(SKZT) on nitric oxid(NO) and superoxide dismutase(SOD) level of the located soft tissue in rat model of acute soft tissue injury. Method: Model of acute soft tissue injury in rats was made,then they were divided into groups and given with drugs.Such dynamic items as index numbers of injury symptoms,parameters of histological anatomy and morphometry were investigated,and changes of NO and SOD in soft tissue were determined. Result: SKZT Plaster had favorable improvement on rat models of acute soft tissue injury,drug group was super to control group on index numbers of injury symptoms and histological anatomy recovery.SKZT Plaster could significantly decrease the elevation of NO in injured soft tissue and increase the contents of SOD in injured soft tissue in a dose-dependent way. Conclusion: SKZT Plaster could increase the level of SOD and inhibit the elevation of NO in injured soft tissue,this may be one of the mechanisms that SKZT Plaster can be used to treat acute soft tissue injury.