| Bax基因在紫杉醇诱导HL-60细胞凋亡过程中作用的研究 |
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| 引用本文： | 尤学芬,谢玉娟,丁润生,陆德炎,姚登福. Bax基因在紫杉醇诱导HL-60细胞凋亡过程中作用的研究[J]. 中国交通医学杂志, 2007, 21(2): 119-120,125 |
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| 作者姓名： | 尤学芬 谢玉娟 丁润生 陆德炎 姚登福 |
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| 作者单位： | 南通大学附属医院血液研究室,江苏,226001;南通大学附属医院血液研究室,江苏,226001;南通大学附属医院血液研究室,江苏,226001;南通大学附属医院血液研究室,江苏,226001;南通大学附属医院血液研究室,江苏,226001 |
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| 摘 要： | 目的:观察bax基因在紫杉醇诱导HL-60细胞凋亡过程中的表达。方法:将不同浓度的紫杉醇作用于HL-60细胞,用RT-PCR法检测药物孵育前后bax基因的表达水平。结果:在紫杉醇诱导HL-60细胞凋亡过程中bax基因的表达水平上升,并显示剂量效应。结论:bax基因参与紫杉醇诱导HL-60细胞凋亡的基因调控。
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| 关 键 词： | 紫杉醇 细胞凋亡 bax基因 HL-60 |
| 文章编号： | 1006-2440（2007）02-0119-02 |
| 收稿时间： | 2007-01-31 |
| 修稿时间： | 2007-01-31 |
Study of bax gene in taxol-induced apoptosis in HL-60 cell line |
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| YOU Xuefen,XIE Yujuan,DIN Runsheng,LU Deyan,YAO Dengfu. Study of bax gene in taxol-induced apoptosis in HL-60 cell line[J]. Chinese Medical JOurnal of Communications, 2007, 21(2): 119-120,125 |
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| Authors: | YOU Xuefen XIE Yujuan DIN Runsheng LU Deyan YAO Dengfu |
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| Affiliation: | Research Section of Hematology,Affiliated Hospital,Nantong University,Jiansu 226001 |
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| Abstract: | Objective:To observe whether antimicrotubular drug taxol might induce apoptosis in HL-60 cell line and to study the role of bax gene in the process. Methods:(1)Different concentrations of taxol were used to treat HL-60 cells and the effects of dose were observed;(2)Bax gene mRNAs were studied by RT-PCR technique. Results:(1)Taxol could inhibit HL-60 cell growth within a certain range of treating dose;(2)Apoptosis was induced in relation to dose ;(3)The expression of bax mRNAs was increased after taxol treatment. Conclusions:Taxol can induce HL-60 cell apoptosis.It might provide a theoretical basis for clinical treatment.Bax gene participate in the taxol induced apoptosis of HL-60 cells. |
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| Keywords: | taxol apoptosis bax gene cell line HL-60 |
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