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| BDNF对脊神经根结扎大鼠脊髓背角星形胶质细胞活化的影响 | |
| 引用本文： | 汪静,张昕,杜冬萍,江伟.BDNF对脊神经根结扎大鼠脊髓背角星形胶质细胞活化的影响[J].上海交通大学学报(医学版),2011,31(11):1578-1582. |
| 作者姓名： | 汪静 张昕 杜冬萍 江伟 |
| 作者单位： | 1. 上海交通大学附属第六人民医院疼痛科,上海200223;上海交通大学附属第六人民医院麻醉科,上海200223 2. 上海交通大学附属第六人民医院疼痛科,上海,200223 3. 上海交通大学附属第六人民医院麻醉科,上海,200223 |
| 摘 要： | 目的观测脊神经根结扎(SNL)大鼠脊髓背角中脑源性神经营养因子(BDNF)与星形胶质细胞活化标记蛋白胶质细胞纤丝酸性蛋白(GFAP)表达的变化,以及BDNF清除剂TrkB/Fc对GFAP表达和疼痛的影响。方法取18只SD大鼠,随机分为空白对照组、假手术组和SNL组(n=6)。分别于术前、术后第1～6天测定大鼠50%机械缩爪阈值(50%PWT),末次测定后取腰膨大处脊髓背角经Western blotting检测GFAP和BDNF的表达。另取18只SD大鼠,随机分为对照组、SNL+安慰剂组和SNL+TrkB/Fc组(n=6),其中对照组仅行鞘内置管术;SNL+安慰剂组和SNL+TrkB/Fc组分别在行SNL术前,予鞘内注入PBS或TrkB/Fc 10μL,术后1次/d×6 d,每次注药前1 h测定50%PWT,末次给药后1 h取大鼠腰膨大处脊髓背角经Western blotting检测GFAP的表达。结果术后第1～6天,SNL组手术同侧后肢50%PWT均较术前基础值显著下降(P<0.01);术后第6天,SNL组手术同侧脊髓背角BDNF和GFAP表达均较空白对照组显著升高(P<0.01);SNL+TrkB/Fc组大鼠手术同侧50%PWT与基础值比较无明显变化(P>0.05),手术同侧脊髓背角GFAP表达与对照组比较差异无统计学意义(P>0.05)。结论 BDNF活化的脊髓星形胶质细胞对SNL诱发的神经病理性疼痛具有调控作用。 |
| 关 键 词： | 脑源性神经营养因子 胶质细胞纤丝酸性蛋白 星形胶质细胞 神经病理性疼痛 |
Effects of BDNF on activation of spinal dorsal horn astrocytes in rats with spinal nerve ligation |
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| WANG Jing,ZHANG Xin,DU Dong-ping,JIANG Wei.Effects of BDNF on activation of spinal dorsal horn astrocytes in rats with spinal nerve ligation[J].Journal of Shanghai Jiaotong University:Medical Science,2011,31(11):1578-1582. | |
| Authors: | WANG Jing ZHANG Xin DU Dong-ping JIANG Wei |
| Institution: | WANG Jing1,2,ZHANG Xin1,DU Dong-ping1,JIANG Wei2(1.Pain Management Center,2.Department of Anesthesiology,the Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200233,China) |
| Abstract: | Objective To observe the changes of expression of brain-derived neurotrophic factor(BNDF) and glial fibrillary acidic protein(GFAP) in rats with spinal nerve ligation(SNL),and investigate the effects of TrkB/Fc,BDNF inhibitor on expression of GFAP and pain.Methods Eighteen SD rats were randomly divided into blank control group,sham operation group and SNL group(n=6).Values of 50% paw withdrawal threshold(50% PWT) were measured before surgery and on six consecutive days after surgery.Spinal dorsal horn of th... |
| Keywords: | brain-derived neurotrophic factor glial fibrillary acidic protein astrocyte neuropathic pain |
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