| 三种方法对大鼠视网膜固定效果的比较研究 |
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| 引用本文： | 李晶晶,朱鸿,施彩虹. 三种方法对大鼠视网膜固定效果的比较研究[J]. 上海交通大学学报(医学版), 2011, 31(8): 1105-1107. DOI: 10.3969/j.issn.1674-8115.2011.08.013 |
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| 作者姓名： | 李晶晶 朱鸿 施彩虹 |
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| 作者单位： | 上海交通大学医学院附属第三人民医院眼科,上海,201900 |
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| 基金项目： | 上海市科学技术委员会(10ZR1418500)~~ |
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| 摘 要： | 目的研究三种不同方法对大鼠视网膜石蜡切片的固定效果。方法根据离体眼球的不同固定方法,将9只SD大鼠随机分为A组(改良固定液固定,n=3)、B组(4%多聚甲醛固定,n=3)和C组(4%多聚甲醛心脏灌注法固定,n=3)。24 h后制作标本进行苏木精-伊红染色,分析比较固定效果。结果 A组固定的眼杯不变形,B组固定的有少许变形,C组固定的基本不变形。显微镜观察显示A组眼球视网膜各层组织结构完整,B组和C组视网膜均有断层、分离和脱片等现象。结论改良固定液固定眼球的方法优于单纯4%多聚甲醛和4%多聚甲醛心脏灌注固定的方法,适用于视网膜石蜡切片的后期研究。
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| 关 键 词： | 眼球固定液 石蜡切片 大鼠视网膜 |
Outcomes of rat retina fixation with three different methods |
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| LI Jing-jing,ZHU Hong,SHI Cai-hong. Outcomes of rat retina fixation with three different methods[J]. Journal of Shanghai Jiaotong University:Medical Science, 2011, 31(8): 1105-1107. DOI: 10.3969/j.issn.1674-8115.2011.08.013 |
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| Authors: | LI Jing-jing ZHU Hong SHI Cai-hong |
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| Affiliation: | LI Jing-jing,ZHU Hong,SHI Cai-hong(Department of Ophthalmology,the Third People's Hospital,Shanghai Jiaotong University School of Medicine,Shanghai201900,China) |
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| Abstract: | Objective To investigate the outcomes of fixation of rat retina paraffin sections with three different methods.Methods Nine SD rats were randomly divided into group A(retina fixation with improved solution,n=3),group B(retina fixation with 4% paraformaldehyde,n=3) and group C(retina fixation with cardiac perfusion of 4% paraformaldehyde,n=3).Paraffin sections were prepared 24 h after retina fixation,HE staining was performed,and the outcomes of fixation were compared. Results There was no eyecup deformation... |
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| Keywords: | eyeball fixation solution paraffin section rat retina |
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