三种反义c-myc RNA对血管平滑肌细胞凋亡的影响

为探讨反义寡脱氧核苷酸对大鼠肝细胞载脂蛋白Ｂ基因的表达是否具有调控作用，并探索其作用机制。以合成的载脂蛋白Ｂ基因正、反义寡脱氧核苷酸加入培养的肝细胞，测量载脂蛋白Ｂ１００含量；用反转录聚合酶锭反应评价载脂蛋白Ｂ基因的表达。结果发现，载脂蛋白Ｂ基因反义宜脱氧核苷酸可明显下调载脂蛋白Ｂ１００的ｍＲＮＡ表达，５、１０、１５和２０μｍｏｌ／Ｌ的反义寡脱氧核苷酸，可分别降低肝细胞载脂蛋白Ｂ深度的２６．６％、

The Inhibitory Effect of Antisense Oligodeoxynucleotides on Apolipoprotein B Expression in Liver Cells of Rat

Aim In order to see the effects of continued and stable expressive antisense c-myc on the apoptosis of vascular smooth muscle cell(VSMC) . Methods Three recombinant retroviral expressive vectors,aM1,aM2 and aM3,which respectively loading reverse fragments of exon 1,2 and 3,were transferred into rat artery SMC and assayed 1 month after their stable expression. Results Apoptosis ratioes of aM3 group were increased to 360%,while those of aM1 and aM3 groups were significantly decreased. In addition,SMC transfected with aM1 and aM3 (especially aM3),emerged morphological features of apoptosis and autophagy,while aM2 commonly appeared bunches of myofilament in electron microscopy. Conclusion Stable expression of antisense c-myc can induce SMC into apoptosis in vitro, and aM3 has the most powerful ability to induce apoptosis and autophagy.