Antinociceptive and Antihyperalgesic Activity of a Traditional Maya Herbal Preparation Composed of Pouteria Campechiana,Chrysophyllum Cainito,Citrus Limonum,and Annona Muricata

Preclinical Research

The purpose of this work was to assess the antinociceptive and antihyperalgesic properties of an herbal preparation, composed of four vegetal species: Pouteria campechiana (P. campechiana ), Chrysophyllum cainito (C. cainito ), Citrus limonum (C. limonum ), and Annona muricata (A. muricata ), that is commonly used in combination (PCCA) in traditional Mayan medicine for the treatment of diabetes and pain. An ethanolic extract of PCCA was prepared at a ratio of 1:1:1:1 for each plant. The systemic antinociceptive effect of PCCA extract (50–600 mg/kg, p.o.) was dose‐dependent in the rat formalin (1%) producing 66% antinociceptive response at 400 mg/kg, p.o. A concentration‐dependent antinociceptive effect of the PCCA extract (20–160 mg/paw) was also demonstrated in the rat capsaicin (0.2%) test. The PCCA extract (100–400 mg/kg, p.o.) had antihyperalgesic effects in alloxan diabetic rats. These findings demonstrate the antinociceptive and antihyperalgesic effects of PCCA and supports the use of the plant extracts in Mayan folk medicine. Drug Dev Res 78 : 91‐97, 2017. © 2017 Wiley Periodicals, Inc.