犬种布鲁氏菌抗原组分及其免疫原性

ANTIGENIC FRACTIONS OF BRUCELLA CANIS AND THEIR IMMUNOGENICITY

A crude complex o{ cell wall, polysaccharide and protein was prapared by salt extraction from X-86-1 strain of Brucella cants isolated in Xinjiang in 1986. The extracts were divided into 2 groups-the Y-rays treated group and the untreated group and inoculated subsutaneaously to healthy guinea pigs respectively. Thirty days after the inoculation, protective immunity was induced against the challenge exposure each by 1000 viable bacteria of homologous strain with the protection rates of 56% and 60% for the 2 groups of extracts respectively. From the control group, however, a bacteria recovering rate of 96% was obtained, showing significant difference (P<0.01) from the experimental groups. Results indicated that fractions of Brucella canis in this study contained effective antigenic components and presented immunogenicity and protective capability to a certain extent.