米诺环素对实验性自身免疫性脑脊髓炎大鼠轴索损伤的影响

目的:探讨米诺环素对实验性自身免疫性脑脊髓炎(EAE)大鼠轴索损伤的影响。方法:以豚鼠全脊髓匀浆(GPSCH)免疫大鼠建立EAE模型。治疗组给予米诺环素而对照组和模型组给予生理盐水灌胃;免疫组化检测β-淀粉样前体蛋白(β-App)的表达。结果:细胞数,脊髓模型组高于治疗组而对照组最低(P<0.05),大脑和脑干模型组高于对照组(P<0.05);平均光密度,脊髓模型组高于另两组(P<0.05),大脑和脑干模型组高于治疗组而对照组最低(P<0.05)。结论:米诺环素可以抑制β-App的表达,可能对EAE轴索损伤有一定保护作用。

Effects of minocycline on axonal injury in rats with experimental autoimmune encephalomyelitis

Objective To investigate the effects of minocycline on axonal injury in rats with experimental autoimmune encephalomyelitis(EAE).Methods EAE was induced in rats by immunization with guinea pig spinal cord homogenate(GPSCH),and then the rats were divided into treatment group,model group,and control group.Rats in treatment group were given minocycline,while rats in the other two groups were given normal saline by gavage.The expression of β-amyloid precursor protein(β-App) was estimated by immunohistochemical method.Results The positive cells in spinal cord were higher in model group than those in the other two groups(P < 0.05),and those in brain and brain stem were significantly higher in model group than those in control group(P < 0.05).The average density in spinal cord,brain,and brain stem in model group were significantly higher than those in the other two groups(P < 0.05).Conclusions Minocycline can regulate the expression of β-App and may have protective effects on axonal injury in EAE.