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| 重组腺病毒介导的绿色荧光蛋白基因转染体外培养的人气道平滑肌的实验研究 | |
| 引用本文： | 蓝海兵,高杨,罗雅玲,钟浩海,张达成,任敦强,赖文岩.重组腺病毒介导的绿色荧光蛋白基因转染体外培养的人气道平滑肌的实验研究[J].实用医学杂志,2011,27(2):171-173. |
| 作者姓名： | 蓝海兵 高杨 罗雅玲 钟浩海 张达成 任敦强 赖文岩 |
| 作者单位： | 1. 南方医科大学南方医院呼吸内科,广州市,510515 2. 广东省惠州市中心人民医院,516001 3. 广州市呼吸疾病研究所,510120 4. 南方医科大学南方医院心血管内科实验室,广州市,510515 |
| 基金项目： | 国家自然科学基金，广东省自然科学基金 |
| 摘 要： | 目的:研究重组腺病毒载体介导的绿色荧光蛋白基因转染体外培养的人气道平滑肌(HASMCs)的方法。方法:用组织贴块原代培养传代至3～8代的HASMCs,重组绿色荧光蛋白基因的腺病毒载体(Ad-GFP)以不同的感染复数(multiplicity of infection,MOI)转染,荧光显微镜和流式细胞术检测其转染效率,CCK-8法检测其对细胞的毒性。结果:随着MOI增高,转染效率增高。MOI=100时Ad-GFP对HASMCs的感染效率可达(96±0.32)%,以感染后48h达高峰,持续7d仍有绿色荧光表达;转染后的细胞与未转染细胞相比,细胞毒性无统计学差异(P>0.05)。结论:重组腺病毒载体介导的绿色荧光蛋白基因Ad-GFP能高效转染HASMCs,转染后对细胞无毒性,是一种安全的基因载体。 |
| 关 键 词： | 转染 人气道平滑肌 重组腺病毒 绿色荧光蛋白 |
Recombinant adenovirus vector mediated transfection of green fluorescent protein gene into human airway smooth muscles in vitro |
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| LAN Hai-bing,GAO Yang,LUO Ya-ling,ZHONG Hao-hai,ZHANG Da-cheng,REN Dun-qiang,LAI Wen-yan.Recombinant adenovirus vector mediated transfection of green fluorescent protein gene into human airway smooth muscles in vitro[J].The Journal of Practical Medicine,2011,27(2):171-173. | |
| Authors: | LAN Hai-bing GAO Yang LUO Ya-ling ZHONG Hao-hai ZHANG Da-cheng REN Dun-qiang LAI Wen-yan |
| Institution: | LAN Hai-bing,GAO Yang,LUO Ya-ling,ZHONG Hao-hai,ZHANG Da-cheng,REN Dun-qiang,LAI Wen-yan.Department of Respiratory Diseases,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
| Abstract: | Objective To evaluate the efficiency of recombinant adenovirus vector mediated transfection of green fluorescent protein gene (GFP) into human airway smooth muscle (HASMCs) in vitro. Methods Three to eight passages of HASMCs cultured by tissue adherence method from the trachea were infected with different multiplicity of infection(MOI). The transfection efficiency was assessed by fluorescence microscope and flow cytometry,and the cell cytotoxicity was measured by CCK-8. Results The transfection efficiency w... |
| Keywords: | Transfection Human airway smooth muscles Recombinant adenovirus Green fluorescent protein |
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