杜仲叶提取物/CPC复合载体对大鼠成骨细胞作用的实验研究

目的:观察不同浓度的杜仲叶提取物及其与CPC复合载体对MC3T3E1大鼠成骨细胞的影响。方法:将培养的MC3T3E1大鼠成骨细胞分为5组:空白组和0、0.152、0.304、0.608 mg/ml不同浓度杜仲叶提取物/CPC复合载体培养组。应用细胞增殖、MTT、扫描电镜法观察不同浓度杜仲叶提取物/CPC复合载体对体外培养成骨细胞48 h的增殖活性及生长、附着的影响。结果:杜仲叶提取物/CPC复合载体可促进成骨细胞的增殖率,尤其以浓度为0.152 mg/ml、0.304 mg/ml时作用较为明显(P<0.05)。结论:杜仲叶提取物/CPC复合载体具有促进成骨细胞的增殖和生长,以及附着的作用。

Effects of calcium phosphate cement/eucommia ulmoides extracts compound on cell proliferation and adhesion of MC3T3E1 osteoblasts in vitro

Objective: To observe the effects of calcium phosphate cement/eucommia ulmoides extracts compound(CPC/EUE) on cell proliferation and adhesion of MC3T3E1 osteoblasts in vitro.Methods: Cultured MC3T3E1 osteoblasts were divided into 5 groups:blank control,CPC/EUE 0,0.152,0.304 and 0.608 mg/ml.3H-TdR and scan electronic microscopy(SEM) were used to observe the cell growth,proliferation and adhesion.Results: The CPC/EUE compound enhanced the cell growth,proliferation and adhesion.The concentrations of 0.152 mg/ml,0.304 mg/ml were more significant in enhancing the cell proliferation rate(P<0.05).Conclusion: CPC/EUE can promote the cell growth,proliferation and adhesion of the osteoblasts.