| 用脱乙酰几丁质制备固定化青霉素酰化酶 |
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| 引用本文： | 侯建明,吴经才.用脱乙酰几丁质制备固定化青霉素酰化酶[J].中国医药工业杂志,1993,24(3):100-103. |
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| 作者姓名： | 侯建明 吴经才 |
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| 作者单位： | 河北中医学院生化教研室 石家庄050091(侯建明)，河北大学生物工程研究所 保定(吴经才，杨秀琴) |
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| 摘 要： | 以青霉素酰化酶基因工程菌为酶源,选用脱乙酰几丁质为载体,试用了4种固定化方法,从4个方面(pH、温度、盐浓度和结合酶量)摸索了固定化条件。结果在适宜条件下获得比活900U/g(干)(NIPAB法)的固定化青霉素酰化酶,回收率可达56%。并测定其酶学性质。
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| 关 键 词： | 青霉素 酰化酶 脱乙酰几丁质 制备 |
IMMOBILIZATION OF PENICILLIN ACYLASE ON CHITOSAN |
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| Authors: | HOU Jian-Ming WU Jing-Caia YANG Xiu-Qina |
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| Institution: | HOU Jian-Ming WU Jing-Cai~a YANG Xiu-Qin~a |
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| Abstract: | Chitosan has been used as the carrier to immobilize the penicillinacylase(PA) which was extracted from E.coli 108/pPAHD1 constructed by DNArecombinant technique.Three different methods and 4 conditions(pH,tempe-rature,NaCl concentration and quantity of PA to be immobilized) have been con-sidered.Under the suitable conditions,the activity of immobilized PA was ashigh as 900 U/g(dry),and the activity retention was around 56%.The propertiesof the immobilized enzyme were determined. |
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| Keywords: | immobilized enzyme penicillin acylase chitosan |
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