日本血吸虫重组膜外蛋白rSj29免疫诊断的初步应用

目的用重组日本血吸虫相对分子质量(Mr)29000膜外蛋白(rSj29)作为诊断抗原,探讨其在血吸虫病流行区免疫诊断的价值。方法在血吸虫病低度流行区芜湖南陵县弋江镇蒲桥和竞河村现场采血394份,同时采集粪样,用改良加藤法(三送九检)或集卵孵化法检查。制备rSj29和成虫粗抗原(AWA),分别用2种抗原进行间接ELISA(单盲法)和间接红细胞凝集试验(IHA)检测血样。结果粪检、IHA、rSj29-ELISA和AWA-ELISA检测结果显示,阳性率分别为4.8%(19/394)、62.2%(245/394)、68.3%(269/394)和89.9%(354/394)。rSj29-ELISA和IHA检测结果差异无统计学意义(χ2=3.2,P0.05),一致率为80.7%(318/394)。IHA、rSj29-ELISA和AWA-ELISA与粪检符合率分别为42.6%(168/394)、36.0%(142/394)和15.0%(59/394)。结论 rSj29间接ELISA法对日本血吸虫病有一定诊断价值。

Evaluation of recombinant 29,000 extra membranous protein for the immunodiagnosis of schistosomiasis japonica

Objective To evaluate the recombinant membranous protein 29 000 （rSj29） of Schistosoma japonicum in the immunodiagnosis of schistosomiasis by indirect ELISA. Methods 394 serum samples collected from two low-endemic villages in Anhui Province were tested by rSj29-ELISA and AWA-ELISA using adult worm antigen (AWA) of S. japonicum. Meanwhile all subjects were each tested by 9 examinations with 3 fecal samples by modified Kato-Katz method, and by indirect hemagglutination assay （IHA）. Results It was found that the positive detection rates of stool examination, IHA, rSj29-ELISA and AWA-ELISA were 4.8% （19/394）, 62.2% （245/394）, 68.3% （269/394）, and 89.9%（354/394）, respectively. There was no statistical difference between rSj29-ELISA and IHA （χ²=3.2, P>0.05） with the coin-cidence of 80.7%（318/394）. The coincidence of IHA, rSj29-ELISA and AWA-ELISA with stool examination was 42.6% （168/394）, 36.0% （142/394）, and 15.0% （59/394）, respectively. Conclusion The indirect ELISA with rSj29 antigen shows similar effect as IHA for the diagnosis of schistosomiasis.