乙二醛对小鼠谷胱甘肽过氧化物酶活力及巯基含量的影响

目的：观察乙二醛对小鼠全血及组织中谷胱甘肽过氧化物酶(GSH-Px)活力及巯基含量的影响，以探讨乙二醛毒作用机制。方法：40只昆明种小鼠随机分为对照组和低、中、高(75，150，300mg／kg)3个剂量染毒组，腹腔注射乙二醛水溶液染毒，染毒24h后测定小鼠全血、肝、脑、心、肾组织中的GSH-Px活力、总巯基(T—SH)、非蛋白巯基(NP—SH)和蛋白巯基(PB—SH)的含量。结果：乙二醛可使小鼠全血和肝、肾GSH-Px活力下降；高剂量组的脑组织及各剂量组全血中T—SH及PB—SH含量增加，而高剂量组的全血、脑和肾脏及各剂量组肝脏的NP—SH含量下降。结论：乙二醛对全血和肝脏、肾脏的GSH-Px活力及对全血、肝、脑、肾中的NP-SH含量的影响主要表现为抑制。对全血及脑中TSH及．PB—SH含量的影响表现为应激性增高。

Effects of Glyoxal in Activity of Antioxidase and the Contents of Sulfhydryl Groups in Blood and Tissues of Mice

Objective To study the probable toxicological mechanism of glyoxal by observing effects of glyoxal on antioxidase and the contents of sulfhydryl groups in mice blood and tissues.Methods 40 mice were divided in random into 1 negative control group and 3 experiment groups injected intraperitoneally with glyoxal of 75 mg/kg (1/8 LD 50 ), 150 mg/kg (1/4 LD 50 )and 300 mg/kg (1/2 LD 50 )respectively.The activity of GSH-Px and content of total sulfhydryl(T-SH), non-protein sulfhydryl(NP-SH)and protein sulfhydryl groups(PB-SH)in blood and tissues of mice were measured 24 h after intoxication.Results The activities of GSH-Px in blood,liver and kidney of mice treated with glyoxal were significantly lower than those of the control.The contents of T-SH and PB-SH in blood of mice treated with all doses of glyoxal and brain of mice treated with high dose of glyoxal were higher than those of the control.In blood,brain and kidney of high-dose groups and in liver in every dose groups the contents of NP-SH were markedly lower than those of the control.Conclusions The activities of GSH-Px in blood,liver and kidney and the contents of NP-SH in blood and tissues of mice were inhibited when exposed to glyoxal.The contents of T-SH and PB-SH in blood and brain have increased after exposed to glyoxal.