高效毛细管电泳测定玄参中6种指标成分的含量

 目的 建立高效毛细管电泳法（HPCE）同时测定不同批次玄参中梓醇、桃叶珊瑚苷、哈巴苷、哈巴俄苷、毛蕊花糖苷和肉桂酸含量的方法。方法 以60 mmol·L^-1硼砂缓冲液(pH 9.3) 为电泳介质，未涂渍标准熔融石英毛细管(75 μm×64.5 cm， 有效长度56 cm)为分离通道，分离电压为20 kV，检测波长为210 nm，毛细管温度为25 ℃，压力进样为5 kPa×6 s。结果 6种指标成分的浓度与峰面积的线性关系良好(r>0.997 1) ；加样回收率为97.05%～103.43%。用此法测定了玄参药材中梓醇、桃叶珊瑚苷、哈巴苷、哈巴俄苷、毛蕊花糖苷和肉桂酸等6种指标成分的含量，得到满意结果。结论 该方法简单、准确，重复性较好，可用于玄参药材内在质量的评价和控制。

Simultaneous Determination of Six Index Components in Scrophularia ningpoensis by HPCE

OBJECTIVE To establish a high performance capillary electrophoresis(HPCE) method for simultaneous determination of catalpol,aucubin,harpagide,harpagoside,acteoside and cinnamic acid in Scrophularia ningpoensis of different batches.METHODS 60 mmol·L-1 sodium borate was used as buffer solution(pH 9.3),uncoated fused silica capillary(64.5 cm×75 μm id,56 cm of effective length) was used,separation voltage was 20 kV,detection wavelength was 210 nm,column temperature was maintained at 25 ℃,and the sample was injected at 50 kPa,6s.RESULTS Six index components showed good linearity(r >0.997 1) in the range of the tested concentration,and the average recoveries of the method were between 97.05%-103.43%.CONCLUSION The method is simple,accurate and reproducible,and can be used for quality control of Scrophularia ningpoensis.