当归散对小鼠低容受性子宫内膜孕激素受体和整合素β_3表达的影响

目的:探讨当归散及其拆方对低容受性子宫内膜PR和整合素β3表达的影响。方法:采用曲普瑞林注射制备小鼠模型,预防性给予受试液,于排卵后24h和48h测定子宫内膜中PR、整合素β3水平。结果:(1)PR的表达:48h时当归散组和归-芩组的PR表达明显上调,达到正常组水平(P0.05);(2)整合素β3的表达:而当归散组和当归黄芩组整合素β3在2个时间点表达无显著差异,与正常组比较差异不显著(P0.05)。结论:当归散和当归-黄芩能抑制曲普瑞林引起的PR过早下调,抑制整合素β3的提前表达,改善子宫内膜容受性。

Effects of DGS on the molecular markers of endometrial receptivity

Objective: To examine the effects of DGS on the expressions of PR and Integrin β3 in mice with low endometrial receptivity induced by COH.Methods: The mice were treated by long-term COH with Triptorelin,and fed with the water solutions of Danggui Powder(DGS),Danggui-Huangqin(GQ) and Chuanxiong-Baishao-Baizhu(QGQ)simultaneously.The expressions of PR and Integrin β3 in the endometrium were detected 24 and 48 hours after ovulation respectively.Results:(1) To 48 hours,the PR expression of Group DGS and Group GQ were significantly increased,and reached the control level(p>0.05).(2) Expression of Integrin β3: No significant increase was observed for either control group or Group DGS relative to that of 24hr and 48hr,and it was no significant difference between the two groups.Conclusions: DGS and GQ could inhibit the decrease of PR expression induced by COH,thereby inhibiting the Integrin β3 over-expression in the endometrium,in order to improve the endometrial receptivity.