HPLC法同时测定人血浆中舍曲林与氯氮平的浓度

目的:建立同时测定人血浆中舍曲林、氯氮平浓度的方法。方法:血浆样品以乙酸乙酯-二氯甲烷(80:20)进行提取并测定,色谱柱为DiamonsilTMC18反相柱,流动相为30mmol.L-1醋酸铵-乙腈(22:78),流速为1.0mL.min-1,柱温为40℃,检测波长为210nm。结果:舍曲林、氯氮平血药浓度分别在20.0～600.0μg.L-1、50.0～1000.0μg.L-1范围内线性关系良好(r分别为0.9986、0.9993),分析方法的检测限为10.0μg.L-1;舍曲林、氯氮平的低、中、高3种浓度平均相对回收率均>95%,提取回收率均>70%。日内、日间RSD均<10%(n=5)。结论:该方法灵敏、准确、简单、快速,可用于临床舍曲林与氯氮平血药浓度监测和药动学研究。

Simultaneous Determination of the Concentration of Sertraline and Clozapine in Human Plasma by HPLC

OBJECTIVE:To establish a method for the simultaneous determination of sertraline and clozapine concentration in human plasma.METHODS:Plasma sample was extracted by acetic ether-methylene chloride(80:20).The determination was performed on DiamonsilTM C18 column with mobile phase consisted of 30 mmol·L-1 ammonioum acetate-acetonitrile(22:78) at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 210 nm and column temperature was 40 ℃.RESULTS:The linear ranges were 20.0～600.0 μg·L-1 for sertralin(r=0.998 6) and 50.0～1 000.0 μg·L-1 for clozapine(r=0.999 3),respectively.The detection limit was 10.0 μg·L-1.The relative recovery rates of sertraline and clozapine in low,middle and high concentrations were all over 95%;the extraction recoveries were more than 70%.The intra-day and inter-day RSDs were less than 10%(n=5).CONCLUSION:The method is sensitive,accurate,simple and rapid,and it can be used for blood concentration monitoring and pharmacokinetics study of sertraline and clozapine in the clinic.