| 脂质体介导入β—神经生长因子基因转染培养肌母细胞的表达研究 |
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| 引用本文： | 毛伯镛 陈俊杰 等. 脂质体介导入β—神经生长因子基因转染培养肌母细胞的表达研究[J]. 华西医科大学学报, 2002, 33(1): 12-14 |
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| 作者姓名： | 毛伯镛 陈俊杰 等 |
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| 作者单位： | [1]四川大学华西医院神经外科，成都610041 [2]华西基础医学与法医学院，重组DNA研究室 |
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| 摘 要： | 目的：了解含β－神经生长因子（β－NGF）基因的表达质粒PSVCEP NGF－CAT在培养肌母细胞中的表达及Lipofect AMINE阳离子脂质体介导的转染效率，方法：将表达质粒PSVCEP NGF－CAT经LipofectAMINE转染至培养成的肌母细胞中，用免疫细胞化学方法检测基因在肌母细胞内的表达。结果：培养肌母细胞在转染6小时吞噬脂质体最显著，转染48小时后约40％的肌母细胞胞质内有β-NGF基因表达。结论：含β-NGF基因在表达质粒PSVCEP NGF－CAT可以在培养肌母细胞中表达，脂质体转染的方法简便，有效。
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| 关 键 词： | β－神经生长因子 基因转染 肌母细胞 脂质体 LipofectAMINE 免疫细胞化学 |
Study on expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT with Lipofect AMINE] |
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| Boyong Mao,Yanhui Liu,Junjie Chen,Ruohan Wang,Yongzhong Cheng,Lida Gao,Min He,Shengfu Li. Study on expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT with Lipofect AMINE][J]. Journal of West China University of Medical Sciences, 2002, 33(1): 12-14 |
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| Authors: | Boyong Mao Yanhui Liu Junjie Chen Ruohan Wang Yongzhong Cheng Lida Gao Min He Shengfu Li |
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| Affiliation: | Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China. |
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| Abstract: | OBJECTIVE: To gain an understanding of the expression of beta-NGF in myoblasts transfected by PSVCEP NGF-CAT and the transfecting efficiency of Lipofect AMINE. METHODS: PSVCEP NGF-CAT was delivered into cultured myoblasts by Lipofect AMINE. Expression of beta-NGF in the transfected myoblasts was detected by immunocytochemistry. RESULTS: Lipofect AMINE was evidently engulfed by myoblasts at the 6th hour; about 40% myoblasts could be detected with the expression of beta-NGF by immunocytochemistry at the 48th hour. CONCLUSION: PSVCEP NGF-CAT can express beta-NGF in cultured myoblasts, and Lipofect AMINE is a convenient vector for gene transfection with simplicity and efficacy. |
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