脂质体转染survivin反义寡核苷酸对K562细胞增殖及凋亡的作用

目的:观察脂质体转染survivin反义寡核苷酸(ASODN)对白血病细胞K562的增殖及凋亡的影响。方法:人工合成survivin硫代ASODN,通过脂质体转染K562细胞,倒置显微镜下观察细胞形态学变化;用MTT法观察转染前后对细胞增生的影响;用细胞凋亡检测法(POD)观察细胞凋亡;RT-PCR检测survivin mRNA的表达。结果:(1)survivin ASODN抑制K562细胞增殖呈时间和剂量依赖性;(2)survivin ASODN可诱导K562细胞的凋亡,凋亡指数呈浓度依赖性;(3)survivinASODN处理组K562细胞survivin mRNA的表达水平显著下降。结论:脂质体转染survivin ASODN能够明显抑制K562细胞增殖,且能诱导细胞凋亡。survivin可能成为肿瘤基因治疗的一个新靶点。

Effect of liposomal transfection of survivin antisense oligonucleotide on K562 cell proliferation and apoptosis

Objective:To explore the effect of liposomal transfection of survivin antisense oligodeoxynucleotide(ASODN) on leukemic cell lines K562 cell proliferation and apoptosis.Methods:Anti-survivin phosphorothioate ASODN was synthesized and transfected into K562 cells by liposome,cell morphological transformation was observed with inverted microscope;The effect on cell proliferation was examined by MTT assay. Cell apoptosis was ditermined in situ cell apoptosis detection kit(POD). Survivin mRNA expression was determined by RT-PCR.Results:(1)Survivin ASODN inhibited K562 cells proliferation in a dose and time dependent manner. (2)Survivin ASDON can induce the cell apoptosis and the apoptotic index is in a dose dependent manner. (3)The expression of survivin mRNA significantly decreased after treatment with surviving ASODN.Conclusion:Survivin ASODN is able to inhibit the proliferation of leukemic cells lines K562 cell and induce the cell apoptosis, which might provide a new target for tumor gene therapy.