Genomic characterization of an enterovirus 97 strain isolated in Shandong,China |
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Authors: | Zexin Tao Ning Cui Aiqiang Xu Haiyan Wang Lizhi Song Yan Li Guifang Liu Yao Liu Lei Feng |
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Institution: | (1) Division of EPI, Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, No. 16992 Jingshi Road, Jinan, 250014, People’s Republic of China;(2) Department of Preventive Medicine, College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, No. 4655 Daxue Road, Changqing District, Jinan, 250355, People’s Republic of China; |
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Abstract: | The genomic characterization of human enterovirus 97 (EV97) strain isolated from an acute flaccid paralysis case in Shandong
province, China in 1999, is described. The strain, designated as 99188/SD/CHN/1999/EV97 (abbreviated as 99188), had a genome
of 7394 nucleotides. Compared with other EV97 strains, it had 81.3–83.3% nucleotide similarity and 94.0–95.4% amino acid similarity
in VP1 coding region, and it had 81.4% complete genomic similarity with prototype strain BAN99-10355. The most striking feature
was the deletion of 18 nucleotides in the 3′ end of VP1 coding region, combined with two deletions and one insertion in 5′
and 3′ untranslated regions. All these findings demonstrated the strain 99188 had a distant genetic relationship with other
EV97 strains. In the phylogenetic trees generated from VP1 and 3D sequences of human enterovirus species B (HEV-B), the lineages
of strain 99188 were not congruent, suggesting the event of recombination. Similarity plot analysis further provided the evidence
of recombination with other strains of HEV-B in P2 and P3 coding region. This is the first finding of EV97 in China and the
third genomic sequence of EV97 reported. |
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