| 香加皮不同提取部位体外抗肿瘤活性实验研究 |
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| 引用本文： | 刘洋,刘虹,王小莹,张晗,高秀梅. 香加皮不同提取部位体外抗肿瘤活性实验研究[J]. 天津中医药, 2008, 25(2): 153-156 |
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| 作者姓名： | 刘洋 刘虹 王小莹 张晗 高秀梅 |
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| 作者单位： | 1. 天津中医药大学中医药研究院现代中药发现与制荆技术教育部工程研究中心,天津,300193
2. 天津中医药大学,天津,300193 |
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| 基金项目： | 天津市高等学校科技发展基金计划项目 , 天津市科技创新专项资金项目 |
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| 摘 要： | [目的]筛选香加皮的抗肿瘤活性部位.[方法]用大孔吸附树脂柱层析法对香加皮的和60%乙醇回流提取物进行分段处理,利用四甲基偶氮唑盐(MTT)法测定各提取部位体外抗肿瘤活性.[结果]70%乙醇洗脱部位对体外肿瘤细胞表现出抑制活性,并在测定浓度范围内呈现出良好剂量依赖性.95%乙醇洗脱部位的抑制作用较弱.而水洗脱物和20%乙醇洗脱物则未呈现出抗肿瘤活性.在各提取部位中70%乙醇提取物活性最强.[结论]70%乙醇提取部位是香加皮主要的体外抗肿瘤活性部位.
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| 关 键 词： | 香加皮 抗肿瘤活性 四甲基偶氮唑盐 香加皮 不同提取部位 肿瘤活性 实验 研究 different extract activity 体外抗肿瘤 皮主 乙醇提取物 水洗脱 抑制作用 剂量依赖性 浓度范围 抑制活性 表现 肿瘤细胞 醇洗 结果 |
| 文章编号： | 1672-1519(2008)02-0153-04 |
| 收稿时间： | 2007-09-30 |
| 修稿时间： | 2007-09-30 |
Antitumor activity of extract from different parts of Periploca Sepium Bge. |
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| LIU Yang,LIU Hong,WANG Xiao-ying. Antitumor activity of extract from different parts of Periploca Sepium Bge.[J]. Tianjin Journal of Traditional Chin Medicine, 2008, 25(2): 153-156 |
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| Authors: | LIU Yang LIU Hong WANG Xiao-ying |
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| Affiliation: | Research College of TCM, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of TCM, Tianjin 300193, China;Tianjin University of TCM, Tianjin 300193, China;Tianjin University of TCM, Tianjin 300193, China;Tianjin University of TCM, Tianjin 300193, China;Tianjin University of TCM, Tianjin 300193, China |
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| Abstract: | [Objective] To screen the part of Periploca Sepium Bge.(PSBF) with antitumor activity.[Methods] The extract of Periploca Sepium Bge.with 60% ethanol was treated in the way of macroporous adsorptive resins column chromatography to afford different fractions.The antitumor activities of the different fractions in vitro were determined by MTT assay.[Results] The 70% ethanol elution(PSBF-C) showed strong inhibitory effect on growth of cancer cells with significant dosage-dependence.The 95% ethanol elution(PSBF-D) showed low antitumor activity in vitro.And the most sensitive tumor cell lines to PSBF-C and PSBF-D were human hepatocellular carcinoma SMMC-7721 cell line.Whereas the fraction of 20% ethanol/H2O elution showed no antitumor activity.[Conclusions] The extract of 70% ehanol elution was the principal antitumor fraction from Periploca Sepium Bge. |
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| Keywords: | Periploca Sepium Bge Antitumor activity MTT |
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