白芥子提取物抑制前列腺增生的实验研究(Ⅰ)

目的 :研究白芥子提取物对实验性小鼠前列腺增生的抑制作用及有效药用部位。方法 :采用丙酸睾酮诱导的去势雄性小鼠前列腺增生为动物模型。以 60%乙醇提取制得的白芥子总提取物和溶剂极性依次递增分离法 (乙醚-乙醇-水连续加热回流提取 )制得的分段提取物Ⅰ ,Ⅱ ,Ⅲ为药效研究对象。结果 :白芥子总提取物、白芥子分段提取物Ⅰ和Ⅱ均能显著抑制由丙酸睾酮诱发的去势小鼠前列腺增生 ,明显降低小鼠包皮腺湿重和血清酸性磷酸酶活力 ,而提取物Ⅲ无活性。结论 :白芥子总提取物、分段提取物Ⅰ和Ⅱ能显著抑制外源激素诱导的去势小鼠前列腺增生 ,表现出抗雄激素活性。白芥子分段提取物Ⅰ ,Ⅱ表现出与总提取物相当的药效 ,提示这两部分提取物含有白芥子抗前列腺增生的活性成分。

An experimental study(I) on the inhibition of prostatic hyperplasia with extract of seeds of Brassica alba]

OBJECTIVE: To study the effective fraction of the extract of seeds of Brassica alba, which inhibits experimental mice prostatic hyperplasia. METHOD: An experimental model of prostatic hyperplasia of castrated male mice induced by testosterone propionate was made. Fractions I, II and III were prepared by extracting the seeds of Brassica alba successively with ether, ethanol and water under reflux. Total extract was prepared by extracting the seeds of Brassica alba with 60% ethanol under reflux. The total extract and the three fractions were used to test the activities. RESULT: Total extract, fractions I and II could not only significantly inhibit mice prostatic hyperplasia induced by testosterone propionate and activity of serum acid phosphatase, but also decrease wet weight of preputial glands, while fraction III is inactive. CONCLUSION: Extract from seeds of Brassica alba can significantly inhibit mice prostatic hyperplasia induced by exterior hormone, possessing an activity of anti-androgen. Fractions I and II show an equivalent activity of total extract, which indicate that these fractions contain active components of seeds of Brassica alba which can inhibit prostatic hyperplasia.