Expression of SAG-1 of Toxoplasma gondii in transgenic mice |
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Authors: | S. Seng M. Yokoyama R. Suzuki Y. Maki M. Kato C. Lim B. Zayatiin N. Inoue X. Xuan I. Igarashi H. Nagasawa K. Fujisaki T. Mikami N. Suzuki Y. Toyoda |
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Affiliation: | (1) The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan e-mail: nrcpmi@obihiro.ac.jp Tel.: +81-155-495644; Fax: +81-155-495643, JP;(2) Reproductive Engineering Section and Experimental Animal Center, Mitsubishi Kasei Institute of Life Science, Tokyo 194-8511, Japan, JP;(3) Nihon Genosys Biotechnologies, Ishikari, Hokkaido 080, Japan, JP |
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Abstract: | We describe the expression of SAG-1 cDNA in B6C3F1 mice by microinjecting a 3.3 kbp DNA fragment, consisting of the cytomegalovirus enhancer-chicken β-actin hybrid promoter and SAG-1 into the pronucleus of a fertilized egg at the one-cell stage. Offspring derived from this microinjection were analyzed for the integration and functional expression of the SAG-1 transgene. Steady-state expressions of both the mRNA for SAG-1 and SAG-1 protein product were detected in the brain, thymus, spleen and liver. Approximately 50% of F1 and F2 progeny inherited the SAG-1 transgene from SAG-1 transgenic mice in Mendelian fashion. These results indicated that SAG-1 transgenic lines were established. Transgenic mice harboring the SAG-1 gene will contribute a critical tool of defining the molecular mechanisms of SAG-1 in pathogenesis and host immune response. Received: 28 September 1999 / Accepted: 14 October 1999 |
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