| 瓜蒌注射液对兔血管平滑肌细胞增殖细胞核抗原表达的影响 |
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| 引用本文： | 李自成,常青. 瓜蒌注射液对兔血管平滑肌细胞增殖细胞核抗原表达的影响[J]. 中国病理生理杂志, 2000, 16(6): 516-518 |
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| 作者姓名： | 李自成 常青 |
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| 作者单位： | 1. 暨南大学医学院第一附属医院心内科, 广东 广州 510630;
2. 暨南大学医学院组织学与胚胎学教研室 |
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| 基金项目： | 国务院侨办重点基金，广东省中医药管理局科研基金资助 |
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| 摘 要： | 目的:观察瓜蒌注射液对血管平滑肌细胞(SMC)增殖细胞核抗原(PCNA)表达的影响。方法:用免疫组织化学LSAB法检测培养的兔主动脉SMC中PCNA的表达,液闪法测定SMC氚-胸腺嘧啶核苷([3H]-TdR)掺入量,同时测定培养液中超氧化物歧化酶(SOD)、脂质过氧化物(LPO)、前列环素(PGI2)及环磷酸腺苷(cAMP)的含量。结果:瓜蒌注射液能增加SOD活性,降低LPO和升高PGI2、cAMP水平,抑制SMC的[3H]-TdR掺入量和PCNA的表达(P均＜0.05,0.01)。结论:瓜蒌有抑制SMC增殖的作用。
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| 关 键 词： | 瓜蒌 中草药 肌 平滑 血管 细胞 抗原 |
| 收稿时间： | 1999-09-06 |
Effect of trichosanthes injection on expression of proliferating cell nuclear antigen of vascular smooth muscle cell in rabbit |
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| LI Zi-cheng,CHANG Qing. Effect of trichosanthes injection on expression of proliferating cell nuclear antigen of vascular smooth muscle cell in rabbit[J]. Chinese Journal of Pathophysiology, 2000, 16(6): 516-518 |
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| Authors: | LI Zi-cheng CHANG Qing |
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| Affiliation: | Department of Cardiology, The First Affiliated Hospital, Medical College of Jinan University, Guangzhou 510630,China |
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| Abstract: | AIM: To observe the effect of thichosanthes injection on the expression of proliferating cell nuclear antigen (PCNA) of vascular smooth muscle cell (SMC). METHODS: The expression of PCNA of cultured rabbit aortic SMC was examined with LSAB immunohistochemical technique, and [ 3H]-thymidine([ 3H]-TdR) incorporation data of SMC and the contents of superoxide dismutase (SOD), lipid peroxide (LPO), prostacyclin (PGI 2) and cyclic AMP (cAMP) in medium were simultaneously determined. RESULTS: Thichosanthes injection has an effects of increasing SOD activity, decreasing LPO, elevating PGI 2 and cAMP, reducing [ 3H]-TdR incorporation and expression of PCNA (all P< 0 05, P <0 01). CONCLUSION: Thichosanthes could inhibit SMC proliferation. |
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| Keywords: | Thichosanthes Drugs Chinese herbal Muscle smooth vascular Cells Antigens |
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