红景天对卒中后抑郁大鼠海马组织细胞因子干预作用的实验研究

目的探讨红景天对卒中后抑郁(PSD)大鼠海马组织细胞因子的干预作用。方法选用健康雄性SD大鼠,随机分为假手术组、单纯缺血组、PSD组及红景天低、中、高3个剂量组。制作PSD大鼠模型,术后21d内红景天低、中、高剂量组每日以红景天灌胃给药,其余各组以同样给药途径予等剂量的生理盐水,于第1、7、14、21日时进行行为学观察和测试,于第21日时进行海马HE染色观察病理变化,测定海马组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的含量。结果与假手术组比较,各组大鼠海马组织TNF-α、IL-1β含量不同程度增高,而IL-6含量则减少,抑郁的行为学评分下降(P0.01),HE染色示海马神经细胞受损;与单纯缺血组比较,PSD组大鼠海马组织TNF-α、IL-1β含量明显增高,而IL-6含量则明显减少,抑郁的行为学评分明显下降(P0.01),HE染色示海马神经细胞受损明显;与PSD组比较,红景天低、中、高剂量组均能不同程度减少海马组织TNF-α、IL-1β含量,升高IL-6含量,减少海马神经细胞的受损程度,提高抑郁的行为学评分(P0.01),其中红景天中、高剂量组较低剂量组作用更明显(P0.05),但中、高剂量组间无明显差异(P0.05)。结论 PSD大鼠海马组织细胞因子网络稳态发生改变,海马损伤进一步加重;红景天对PSD大鼠海马有保护作用,可能是通过降低致炎性细胞因子的产生而促进抗炎性细胞因子的产生而实现的。

Experimental Study of the Interference of Hongjingtian in Cytokines in Hippocampus of Post Stroke Depression Rats

Objective To study the interference of Hongjingtian in cytoldnes in hippocampus of post stroke depression （PSD） rats. Methods The healthy SD rats were randomly divided into five groups： simple focal cerebral ischemia group, PSD group, Hongjingtian group of three doses [2.5, 5, 7.5 g/（kg · d）]. PSD rats model was established. Hongjingtian group was given Hongjingtian and other groups were given normal saline. The ethological score of depression was evaluated. Pathological changes of hippocampus were observed by HE staining and the content of TNF-α, IL-1β and IL-6 in hippocampus were measured. Results Compared with normal group, the expression of the content of TNF-α and IL-1β of other groups increased, while the content of IL-6 decreased, the ethological score of depression decreased. HE staining of hippocampus showed neurocyte damaged obviously. The difference between them was significant （P 〈 0.01）. Compared with simple focal cerebral ischemia group, the content of TNF-α and IL-1β in model group increased, while the content of IL-6 decreased. The ethological score of depression decreased. HE staining of hippocampus showed neurocyte damaged obviously. The difference between the two groups was significant （P〈0.01）. Compared with model group, Hongjingtian groups decreased the content of TNF-α and IL-1β, increased the content of IL-6, and increased the ethological score of depression, reduced the damaged extent of neurocyte of hippocampus. The difference between the two groups was significant （P 〈 0.01）. The effects of medium-dose and high-dose groups were more obviously compared with the low-close groups （P〈0.05）, but there was no difference between them （P〉0.05）. Conclusion The steady state of network of cytokines in PSD experienced disequilibrium and neurocyte of hippocampus were damaged worse than that in simple focal cerebral ischemla. Hongjingfian has neuroprotective effects on PSD rats, its mechanism may through improving the damaged extent of neurocyte of hippocampus by way of reducing the content of TNF-α, IL-1β and increasing the content of IL-6 in hippocarnpus.