| 甘草多糖对小鼠免疫调节作用的影响 |
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| 引用本文： | 李发胜,赵珏,池晓峰,杨波,燕小梅,刘海日.甘草多糖对小鼠免疫调节作用的影响[J].中国中医药信息杂志,2009,16(6):35-36. |
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| 作者姓名： | 李发胜 赵珏 池晓峰 杨波 燕小梅 刘海日 |
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| 作者单位： | 1. 大连医科大学检验医学院,辽宁,大连,116044
2. 中国人民解放军沈阳军区大连疗养院,辽宁,大连,116023 |
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| 基金项目： | 辽宁省教育厅科技基金 |
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| 摘 要： | 目的观察甘草多糖对小鼠免疫调节作用的影响。方法采用水提醇沉法提取甘草多糖，用卵清蛋白为抗原给小鼠注射后，将48只小鼠随机分为4组，分别为甘草多糖低（50mg／kg）、中（100mg／kg）、高（200mg／kg）剂量实验组及对照组，每组12只。对照组给予生理盐水灌胃，实验组予以不同剂量甘草多糖灌胃，灌服小鼠7d，加强免疫后，检测相应抗体生成水平和血清中γ-干扰素（IFN—γ）生成水平。结果与对照缉比较，甘草多糖中、低剂量组卵清抗体生成水平、IFN—γ激发水平均显著增高（P〈0．05，P〈0．01）。结论甘草多糖对小鼠机体免疫有增强作用。
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| 关 键 词： | 甘草 多糖 免疫调节 γ-干扰素 小鼠 |
Effect of Glycyrrhiza Polysaccharide on Immunomodulation in Mice |
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| Institution: | LI Fa-sheng, ZHAO Jue, CHI Xiao-feng, et al (1.College of Clinical Laboratory, Dalian Medical University, Dalian 116044, China; 2.Dalian Convalescent Hospital of Shenyang Military Region of PLA, Dalian 116023, China) |
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| Abstract: | Objective To study the effects of Glycyrrhiza polysaccharide （GP） on immune function in mice. Methods Polysacchayide was extracted from Glycyrrhiza uralensis by water-extraction-alcohol-precipitation method. Ovalbumin was injected to mice as antigens. The mice were randomly divided into four group： low （50 mg/kg）, middle （100 mg/kg）, high （200 mg/kg） dosage group of GP and normal control group, 12 mice in each group. Four groups were given GP and NS orally for seven days. After the secondary immunization, the level of antibody and the concentration of serum IFN-γ were determined. Results The levels of antibodies and cytokines in middle and low dosage group of GP were all significantly higher than those of control group. Conclusion GP could enhance the immune function of mice. |
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| Keywords: | Glycyrrhizauralensis polysaccharide immunomodulation IFN-γ mice |
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