| 聚合酶链反应法扩增宫颈癌c-Ha-ras癌基因100bp片段 |
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| 引用本文： | 丁晓华,赵文先,杨平,伍欣星,戴天力.聚合酶链反应法扩增宫颈癌c-Ha-ras癌基因100bp片段[J].武汉大学学报(医学版),1990(3). |
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| 作者姓名： | 丁晓华 赵文先 杨平 伍欣星 戴天力 |
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| 作者单位： | 湖北医学院病毒研究所生化室，湖北医学院病毒研究所生化室，湖北医学院病毒研究所生化室，湖北医学院病毒研究所生化室，湖北医学院病毒研究所生化室 |
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| 基金项目： | 本课题系“七·五”国家科研攻关专题资金资助 |
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| 摘 要： | 富颈癌 DNA 在两种特异引物和 DNA 聚合酶的存在下,经35轮循环的变性、退火和链的延伸反应,c-Ha-ras 癌基因100bp 片段被放大约10~6倍。反应产物经琼脂糖或聚丙烯酰胺凝胶电泳可见清晰的扩增带。
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| 关 键 词： | 子宫肿瘤 基因 脱氧核糖核酸聚合酶Ⅰ类 脱氧核糖核酸聚合酶Ⅱ类 电泳 聚丙酰凝胶 |
Amplication of 100 bp Fragment of c-Ha-ras in Cervical Cancer by Polymerase Chain Reaction |
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| Ding Xiaohua.Amplication of 100 bp Fragment of c-Ha-ras in Cervical Cancer by Polymerase Chain Reaction[J].Medical Journal of Wuhan University,1990(3). |
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| Authors: | Ding Xiaohua |
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| Abstract: | This paper described the method with which that 100 bp fragment of the first exon of c-Ha-ras is amplified by the Polymerase chain reaction. The DNA of cervical cancer was denatured, then the primers annealed to the target sequence, and DNA polymerase catalyzed the extention of the primers. After thirty-five cycles, the speific fragment was amplified 10~6 times. Analyzed by agarose gel elec- trophoresis or acrylamide gel electrophoresis, the band of DNA was visible under the UV light. This technique may be used for diagnosis of the c-Ha-ras oncogene and the final reaction mixture may be used for the diagnosis of the c-Ha-ras oneogene and the final reaction mixture maybe used for the anal- ysis of mutation of the c-Ha-ras oncogene. |
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| Keywords: | cervix neoplasms genes DNA polymerase I DNA polymerase Ⅱ |
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