利用末梢血检测肺炎支原体IgM抗体的研究

目的 :研究利用末梢血检测肺炎支原体 (MP)IgM抗体的可行性。方法 :利用ELISA法检测 117例肺炎患儿静脉血及末梢血肺炎支原体IgM抗体 ,对其定性检测结果进行比较 ,并对MPIgM抗体值在 2 0 0～ 30 0 0u/ml范围内的 90例患儿的静脉血和末梢血MPIgM抗体水平进行比较 ,进行直线相关及直线回归分析。结果 :117例患儿静脉血和末梢血MPIgM抗体定性检测结果无显著差异 ,P >0 0 5。 90例患儿末梢血组MPIgM抗体水平 ( 912 5 3± 5 48 45u/ml)显著低于静脉血组 ( 10 2 4 0 2± 6 0 4 90u/ml) ,P <0 0 0 1,但二者具有高度相关关系 ,r =0 975。建立了以末梢血MPIgM抗体值为自变量 (x)的估测静脉血MPIgM抗体值 (y)的回归方程为 :y =46 +1 0 6 8x。结论 :利用末梢血检测肺炎支原体IgM抗体是可行性。

Study on detecting mycoplasma pneumonia IgM antibody with terminal blood

Objective: To study the feasibility of detecting mycoplasma pneumonia (MP) IgM antibody with terminal blood.Methods: Venous blood and terminal blood of 117 sick children were detected respectively for mycoplasma IgM antibody,the result of qualitative analysis was compared.90 MP IgM antibody levels with a range of 200 to 3000 u/ml of terminal blood were compared with that of venous blood.Correlation coefficient was calculated,and regression equation was developed.Results: In this study,the result of qualitative analysis on MP IgM antibody of venous blood and terminal blood was no significant difference (P>0.05).The MP IgM antibody level of terminal blood (912.53±548.45 u/ml) was significantly lower than that of venous blood (1024.02±624.90 u/ml)(P<0.05),but there was a significant correlation(r=0.975).The MP IgM antibody of venous can be estimated by the equation:1.068×terminal blood+46.Conclusion: It is feasible to detect MP IgM antibody with terminal blood.