HPV16,18E6蛋白与p21ras,p53在食管癌组织中表达

采用ＳＰ免疫组化法对５２例食管鳞状细胞癌和３０例食管粘膜慢性炎（对照组）进行高危ＨＰＶ１６、１８Ｅ_６和ｐ２１ｒａｓ、ｐ５３癌基因产物的检测。结果表示：鳞癌组中Ｅ_６的阳性率为６７．３１％，与对照组相比差异有极显著性（Ｐ＜０．００１），其中Ｅ_６与ｐ５３呈双阳性者为５５．７７％（２９／５２）、８９．６６％（２６／２９），显示两者阳性着色出现在部分相同区域同一癌细胞核内（似表明Ｅ_６可与ｐ５３结合形成复合物从而导致野生型ｐ５３的降解）。本组ｐ２１ｒａｓ与ｐ５３、ｐ５３与Ｅ_６的阳性表达均具相关性（Ｐ＜０．０５）。提出ＨＰＶ１６、１８感染与本地区食管癌病因学密切相关，Ｅ_６抗体是诊断ＨＰＶ１６、１８感染的良好标记。

Expression of HPV16,18 E_6 protein and p21ras,p53 in the carcinoma of esophagus

The expression of HPV16,18 E_6 and p21ras, p53 oncoprotein in 52 cases of esophageal squamous cell carcinoma and 30 cases of chronic esophagitis were examined with immunohistochemistry.The result showed:67.31%of HPV16,18 E_6 was positive in malignant group,but lower expression in the control group(P<0.001), 55.77%(29/52) were both E_6 and p53 positive and 89. 66%(26/29) stained in the same part of cell nucleus(it seems that binding of p53 with E_6 protein form detectable complexes, which lead to accelerate degradation of wild type p53). Positive expression of p21ras and p53, p53 and E_6 showed a significant correlation respectively (P<0.05).It suggested that high risk HPV16,18 infection was closely related to esophageal carcinogenesis, E_6 antibody is an excellent marker in diagnosis of HPV16,18 infection.