4种癌基因mRNA在胃癌组织中的表达及意义

应用原位分子杂交（ＩＳＨ）技术，检测８８例胃癌组织中Ｋ－ｒａｓ、Ｈ－ｒａｓ、Ｃ－ｍｙｃ和肿瘤转移抑制基因ｎｍ２３（Ｈ１）ｍＲＮＡ．其阳性结果分别为７８．４％、７０％、５８％和３８．６％，胃癌癌旁移行区正常粘膜和正常胃粘膜上皮的阳性率分别为１８．２％、１７％、１９．３％、２１．６％和０、０、０、３／５，与肿瘤区相比较，除ｎｍ２３外差异均非常显著（Ｐ＜０．０１）。Ｋ－ｒａｓ、Ｈ－ｒａｓｍＲＮＡ表达在在细胞膜内侧，Ｃ－ｍｙｃｍＲＮＡ主要表达在细胞核内，ｎｍ２３－Ｈ１ｍＲＮＡ表达在胞浆内。４种癌基因表达与组织学类型无关，ｎｍ２３－Ｈ１的表达与胃癌病人有无淋巴结转移有关（Ｐ＜０．０１）。

Four oncogenes mRNA expression in gastric carcinoma and its significance

The four oncogenes(K-ras,H-ras, C-myc and tumor metastasis inhibitious gene nm23-H1) mRNA was examined in 88 cases by using in situ hybridization (ISH)method. Its positive rate was 78.4%,70%, 58%and 38.6%respectively,The positive rate of gastric transiated region nearby carcinomas and normal gastric mucomembrane nearby carcinomas was 18.2%, 17%, 19.3%, 21.6%and0,0,0,3/5respectively. Comparing with the positive rate of section of tumour, the discrepancy was very obvious(P<0.01) except the nm23 mRNA. K-ras, H-ras mRNA expression was found on intrasurface of tumour cells, and the C-myc mRNA in nuclei of carcinoma cells,the nm23-H1 mRNA in cytoplasm mainly. The expressions of four oncogenes had no correlation with type of histology.But there was information showing the inverse relation beteen the nm23-H1 gene expression and the lymphatic metastasis .