铁皮石斛总黄酮对D-半乳糖所致小鼠衰老模型的影响

目的:观察铁皮石斛总黄酮对D-半乳糖所致小鼠衰老模型的影响.方法:将昆明小鼠随机分为空白组,模型组,抗坏血酸(100 mg·kg-1)组,铁皮石斛总黄酮高、低剂量(400 mg·kg-1、200 mg · kg-1)组.空白组除外,其余各组颈背部皮下注射5％的D-半乳糖连续6周,建立小鼠亚急性衰老模型.采用ELISA法...

Effect of Dendrobium Officinale Flavonoids on Senescent Model Mice Induced by D-galactose

Objective:To observe the effect of flavonoids from Dendrobium officinale in aging mice induced by D-galactose.Methods:Kunming mice were randomly divided into 6 groups:the control group,the model group,the ascorbic acid(100 mg·kg^-1)group as well as Dendrobium officinale flavonoids high and low(400 mg·kg^-1,200 mg·kg^-1).Except for the control group,the other groups were subcutaneously injected with 5%D-galactose for 6 weeks to establish the subacute senescent model.Then put mice to death and determined the activity of superoxide dismutase(SOD),total antioxidant capacity(T-AOC),and content of monochrome display adapter(MDA)in serum with ELISA.SOD,catalase(CAT),ATP activity,and hydroxyproline(HYP),lipofuscin(LF),type I and type III collagen content in the skin of mice was also determined.And the thymus,spleen,liver,and kidney were removed and weighed to detect organ index.Results:Compared with the model group,each flavonoids from Dendrobium group can increase the SOD,T-AOC,and ATP activity levels in the serum and skin of the model animals to varying degrees,reduce the activity of CAT and the content of MDA in serum.It also increases the content of HYP,typeⅠandⅢcollagen in the skin,and reduce the content of LF,at the same time,increases the thymus,spleen,and kidney organ indexes of model animals and decrease the organ indexes of the liver(P<0.05;P<0.01).Conclusion:The flavonoids from Dendrobium officinale have a certain protective effect on the mice skin aging model.