| 高效液相色谱法测定仙茅中仙茅苷的含量 |
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| 引用本文： | 陆惠文,朱炳辉,梁颖康.高效液相色谱法测定仙茅中仙茅苷的含量[J].中国中药杂志,2002,27(3):192-194. |
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| 作者姓名： | 陆惠文 朱炳辉 梁颖康 |
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| 作者单位： | 广东省药品检验所,广东,广州,510180 |
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| 基金项目： | 国家自然科学基金资助项目 ( 2 96 32 0 5 0 ) |
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| 摘 要： | 目的 :建立中药材仙茅中仙茅苷含量的反相高效液相色谱法。方法 :甲醇为溶剂 ,超声提取 ,Sep-PakC18柱净化 ,色谱柱IntertsillODS- 3,流动相甲醇-水-冰醋酸 (45∶80∶1) ,流速 1.0mL·min-1,检测波长 283nm。结果 :平均回收率为 99.2% ,RSD =1.7% (n=5 ) ,6个不同来源的仙茅药材中仙茅苷的含量在 0.11%～0.35%。结论 :可作为仙茅药材的质量控制方法。
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| 关 键 词： | 仙茅 仙茅苷 高效液相色谱法 固相萃取 |
| 文章编号： | 1001-5302(2002)03-0192-03 |
| 收稿时间： | 7/8/2001 12:00:00 AM |
| 修稿时间： | 2001年7月8日 |
Determination of Curculigoside in Crude Medicine Curculigo orchioides by HPLC |
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| LU Hui-wen;ZHU Bing-hui;LIANG Ying-kang.Determination of Curculigoside in Crude Medicine Curculigo orchioides by HPLC[J].China Journal of Chinese Materia Medica,2002,27(3):192-194. |
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| Authors: | LU Hui-wen;ZHU Bing-hui;LIANG Ying-kang |
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| Institution: | Guangdong Provincial Institute for Drug Control, Guangzhou 510180, Guangdong, China. |
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| Abstract: | OBJECTIVE: To establish a method for the determination of curculigoside in Curculigo orchioides, a species of crude medicine. METHOD: The methanol was used as the solvent of exatraction and the curculigoside was extracted from the crude medicine with the method of the ultrasonic vibration. Using Sep-Pak C18 cartridges to purify the solution, the curculigoside was detected by HPLC. Intersil ODS-3(150 mm x 4.6 mm, 5 microns) chromatographic column was used, mobile phase of methanol-water-ice acetic acid (45:80:1) and detect wavelength was set at UV 283 nm. RESULT: The average recovery was 99.2% for the determination of curculigoside, RSD = 1.7% (n = 5). The content range of the curculigoside for 6 kinds of different samples was from 0.11% to 0.35%. CONCLUSION: This method could be used to control the quality of crude medicine C. orchioides. |
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| Keywords: | Curculigo orchioides curculigoside HPLC Solid-Phase extraction |
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