富含DHA的鸡蛋黄和L-半胱氨酸盐酸盐添加物对冷冻猪精液质量的影响

本文旨在研究富含DHA的鸡蛋黄和L-半胱氨酸添加物对冷冻猪精液质量的影响。从5只皮特兰猪种获得15个射精样本，根据冷冻添加物的组成将其分为4组：正常鸡蛋黄组（组1），富含DHA的鸡蛋黄组（组2），添加了5mmol／L半胱氨酸添加物的正常鸡蛋黄（组3），添加了5mmol／L的半胱氨酸添加物的富含DHA的鸡蛋黄（组4）。精液冷冻保存过后在50℃下解冻12秒。之后检测精子直线前进运动，精子生存力，顶体完整性，解冻精液中精子质膜的功能完整性。只添加IL-半胱氨酸（组3）可以提高精子直线前进运动（P〈0．05），与富含DHA的鸡蛋黄一起添加（组4）既能增加直线前进运动（P〈0．05）又能改善顶体完整性（P〈0．01）。富含DHA的鸡蛋黄单独添加对解冻后精液的质量没有任何作用（P〉0．05）。总之，抗氧化的三．半胱氨酸单独或者与富含DHA的鸡蛋黄一起作用可以明显改善解冻后精液质量，尤其是直线前进运动力和顶体完整性。

Effects of DHA-enriched hen egg yolk and L-cysteine supplementation on quality of cryopreserved boar semen

The objective of the present study was to determine the effects of docosahexaenoic acid （DHA）-enriched hen egg yolks and L-cysteine supplementation on the qualities of the cryopreserved boar semen. A total of 15 ejaculates from 5 Pietrain boars were divided into 4 groups according to the compositions of the freezing extenders used, that is, normal hen egg yolk （group Ⅰ）, DHA-enriched hen egg yolk （group Ⅱ）, normal hen egg yolk with 5 mmol L^-1 of cysteine supplementation （group Ⅲ） and DHA-enriched hen egg yolk with 5 mmol L1 of cysteine supplementation （group Ⅳ）. The semen was cryopreserved using controlled rate freezer and was thawed at 50℃ for 12 s. Progressive motility, sperm viability, acrosome integrity and functional integrity of sperm plasma membrane of the post-thawed semen were evaluated. The supplementation of L-cysteine in the freezing extender alone （group Ⅲ） improved progressive motility （P 〈 0.05）, and the supplementation of L-cysteine in combination with DHA-enriched hen egg yolk （group Ⅳ） improved both progressive motility （P 〈 0.05） and acrosome integrity （P 〈 0.01）. The use of DHA-enriched hen egg yolk alone （group Ⅱ） did not enhance any of the post-thawed semen qualities （P 〉 0.05）. In conclusion, the supplementation of antioxidant L-cysteine alone or in combination with DHA-enriched hen egg yolk significantly improved the post-thawed semen qualities, especially progressive motility and acrosome integrity.