AGR2、Lgr5在结直肠癌中的表达及其临床意义

目的：探讨AGR2和Lgr5在人CRC中表达及其与临床病理特征的关系，评价二者联合检测对CRC诊断的意义。方法采用免疫组化SABC法检测70例CRC组织和20例癌旁正常组织中AGR2和Lgr5的表达情况。结果①CRC组织中AGR2阳性表达率较癌旁正常组织显著性增高（68.57％、25.00％，P﹤0.01）。在CRC组织中， AGR2的阳性表达率与肿瘤分化程度与淋巴结转移呈显著相关（P﹤0.05）。②CRC组织中Lgr5阳性表达率较癌旁正常组织显著性增高（74.29％、40.00％，P﹤0.01）。在CRC组织中，Lgr5的阳性表达率与肿瘤分化程度、浸润深度和淋巴结转移有显著相关性（P﹤0.05）。③联合检测AGR2、Lgr5曲线下面积与单独检测比较，差异有统计学意义（P均﹤0.05）。结论 AGR2、Lgr5在CRC的发生、发展过程中发挥了重要作用，两者联合检测有助于对CRC患者肿瘤进展的判断，为CRC患者的分子诊断和治疗提供了理论支持。

Expression and its clinical significance of AGR2 and Lgr5 in colorectal cancer

Objective To investigate the expression and clinical significance of AGR2 and Lgr5 in CRC and the corre-lation with clinical pathologic features. To evaluate the significance with combined detection of the clinical and patho-logical diagnosis of CRC. Methods AGR2 and Lgr5 expression were assessed in 70 cases of CRC and 20 cases of nor-mal tissues near the cancer by immunohistochemistry. Results ①The expression of AGR2 was significantly higher in CRC than that in normal tissue（68.57%vs 25.00%,P〈0.01）. The AGR2 expression in CRC was correlated with the de-gree of tumor differentiation and lymph node metastasis（P〈0.05）.②The expression of Lgr5 was significantly higher in CRC than that in normal tissue （74.29%vs 40.00%, P〈0.01）. The Lgr5 expression in CRC was correlated with the de-gree of tumor differentiation﹑depth of invasion and lymph node metastasis（P〈0.05）. ③The Area Under Curve（AUC） of combined detection of AGR2 and Lgr5 were significantly higher than detection of both alone. the difference was statis-tically significant （P all 〈0.05）. Conclusion AGR2 and Lgr5 played an important role in the occurrence and develop-ment of CRC, the combined detection not only contribute to the judgment of tumor progression, but also provide theo-retical support for molecular diagnosis and treatment of patients with CRC .