Aging and exercise affect the level of protein acetylation and SIRT1 activity in cerebellum of male rats |
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Authors: | Orsolya Marton Erika Koltai Csaba Nyakas Tibor Bakonyi Tania Zenteno-Savin Shuzo Kumagai Sataro Goto Zsolt Radak |
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Institution: | (1) Faculty of Physical Education and Sport Science, Research Institute of Sport Science, Semmelweis University, Alkotas u. 44, TF, Budapest, Hungary;(2) CIBNOR—Planeacion Ambiental y Conservacion, La Paz, Mexico;(3) Graduate School of Human-Environment Studies, Kyushu University, Kasuga, Fukuoka 816-8580, Japan; |
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Abstract: | Aging is associated with a gradual decline in cognitive and motor functions, the result of complex biochemical processes including
pre- and posttranslational modifications of proteins. Sirtuins are NAD+ dependent protein deacetylases. These enzymes modulate the aging process by lysine deacetylation, which alters the activity
and stability of proteins. Exercise can increase mean life-span and improve quality of life. Data from our laboratories revealed
that 4 weeks of treadmill running improves performance in the Morris Maze test for young (4 months, old) but not old (30 months,
old) male rats, and the exercise could not prevent the age-associated loss in muscle strength assessed by a gripping test.
The positive correlation between protein acetylation and the gripping test suggests that the age-dependent decrease in relative
activity of SIRT1 in the cerebellum impairs motor function. Similarly to the acetylation level of total proteins, the acetylation
of ά -tubulin is also increased with aging, while the effect of exercise training was not found to be significant. Moreover,
the protein content of nicotinamide phosphoribosyltransferase, one of the key enzymes of NAD biosynthesis, decreased in the
young exercise group. These data suggest that aging results in decreased specific activity of SIRT1 in cerebellum, which could
lead to increased acetylation of protein residues, including ά-tubulin, that interfere with motor function. |
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