| HPLC标准曲线法测定格列吡嗪控释片的释放度及平均释放速率 |
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| 引用本文： | 窦佳,董莉,戴睿娇.HPLC标准曲线法测定格列吡嗪控释片的释放度及平均释放速率[J].中国医药指南,2013,0(23):50-51. |
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| 作者姓名： | 窦佳 董莉 戴睿娇 |
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| 作者单位： | 窦佳 (大连市食品药品检验所,辽宁大连,116021)； 董莉(大连市食品药品检验所,辽宁大连,116021)；戴睿娇(大连理工大学,辽宁大连,116024)； |
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| 摘 要： | 目的用高效液相色谱标准曲线法测定格列吡嗪控释片的释放度。方法用C18柱;流动相:磷酸盐缓冲液(取磷酸二氢钠13.8g加水溶解并稀释至1000mL,用2mol/L氢氧化钠调pH至6.0)-甲醇(55∶45);检测波长:225nm。采用标准曲线法测定释放度。结果格列吡嗪浓度在1.0~10.0μg/mL范围内与峰面积呈良好的线性关系,r=0.9998。平均加样回收率为100.19%,RSD=0.99%(n=5)。结论本方法灵敏度高、专属性强、准确可靠,较准确的反映了格列吡嗪控释片每4h的释放情况。
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| 关 键 词： | 格列吡嗪控释片 高效液相 标准曲线法 释放度 平均释放速率 |
Determination of the Drug Release and the Average Release Rates of Glipizide Extended Release Tablets by HPLC Standard Curve Method |
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| Dou Jia,Dong Li,Dai Rui-jiao.Determination of the Drug Release and the Average Release Rates of Glipizide Extended Release Tablets by HPLC Standard Curve Method[J].Guide of China Medicine,2013,0(23):50-51. |
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| Authors: | Dou Jia Dong Li Dai Rui-jiao |
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| Institution: | 1 Dalian Institute for Food and Drug Control,Dalian 116021,China;2 Dalian University of Technology,Dalian 116024,China) |
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| Abstract: | Objective A HPLC standard curve method has been established for the determination of the drug release of glipizide extended release tablets.Methods C18 column was used.The mobile phase was phosphate BS（dissolve 13.8g of sodium dihydrogen phosphate in water and dilute to 1000mL,adjust pH to 6.0 with 2mol L-1 sodium hydroxide）-Methonal（55∶45）.The detection wavelength was at 225 nm.The HPLC standard curve method was employed to determine the drug release.Results The linear range of 1.0~10.0μg/mL（r=0.9998） was obtained.The average recovery of add sample was 100.19%,RSD was 0.99%（n=5）.Conclusion The method was of high sensitivity and specificity,accurate,reliable.The method can reflect accurately the drug release of glipizide extended release tablets every 4 hours. |
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| Keywords: | Glipizide extended release tablets HPLC Standard curve method Drug release Average release rates |
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