Tyrosine hydroxylase-like immunoreactive neurons in the olfactory bulb of the snake,Elaphe quadrivirgata,with special reference to the colocalization of tyrosine hydroxylase- and GABA-like immunoreactivities |
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Authors: | T Kosaka K Kosaka I Nagatsu |
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Institution: | (1) Department of Anatomy, Faculty of Medicine, Kyushu University, Higashi-ku, 812 Fukuoka, Japan;(2) Department of Anatomy, School of Medicine, Fujita-Gakuen Health University, Toyoake, 470-11 Aichi, Japan |
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Abstract: | Summary The distribution and structural features of tyrosine hydroxylase-like immunoreactive (TH-LI) neurons were studied in the olfactory bulb of a snake, Elaphe quadrivirgata, by using pre-and post-embedding immunocytochemistry at the light microscopic level. In contrast to rodent olfactory bulbs previously reported, many TH-LI neurons were seen not only in the main olfactory bulb (MOB) but also in the accessory olfactory bulb (AOB). With regard to the TH-like immunoreactivity, there appeared no appreciable differences between MOB and AOB. As in mammalian MOB, the majority of TH-LI neurons were clustered in the periglomerular region and appeared to send their dendritic branches into glomeruli, which as a whole make an intense TH-LI band in the glomerular layer (GML). In the external plexiform/mitral cell layer (EPL/ML) of MOB and AOB as well as in the outer sublamina of the internal plexiform layer (OSL) of AOB, an appreciable number of TH-LI neurons were scattered, extending dendritic processes which appeared to make a loose meshwork. TH-LI neurons in EPL/ML (including OSL) appeared to consist of at least two morphologically different types. The first had a small perikaryon and one or two smooth dendrites which usually extended to GML and were frequently confirmed to enter into glomeruli. The second had a larger perikaryon and 2–3 dendrites which branched into several varicose processes extending in EPL/ML/OSL but appeared not to enter into glomeruli. The TH-like immunoreactivity was rarely seen in the internal plexiform layer and internal granule cell layer. The colocalization of GABA-like and TH-like immunoreactivities was further studied. Almost all TH-LI neurons in both EPL/ ML/OSL and GML contained GABA-like immunoreactivity irrespectively of the type of TH-LI cells.Abbreviations in Figures AOB
accessory olfactory bulb
- MOB
main olfactory bulb
- Hem
hemisphere
- ON
olfactory nerve layer
- VN
vomeronasal nerve layer
- GM
glomerular layer
- EP/M
external plexiform layer/Mitral cell layer
- IP
internal plexiform layer
- IG
internal granular layer
- OS
outer sublamina of the IPL of AOB
- MS
middle sublamina of the IPL of AOB
- IS
inner sublamina of the IPL of AOB |
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Keywords: | Tyrosine hydroxylase Catecholamine GABA Coexistence Olfactory bulb Snake Comparative neuroanatomy |
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