儿童过敏性紫癜与T-细胞亚群研究

目的探讨T-细胞亚群与过敏性紫癜（HSP）及其各临床亚型的关系以明确T-细胞亚群在HSP发病机制中的作用，并通过对HSP各临床亚型患儿的血清补体系列、免疫复合物和免疫球蛋白水平进行对比分析以了解HSP各临床亚型是否在发病机制上存在差异。方法对32例未接受过糖皮质激素及其他免疫抑制治疗的初发HSP患儿用流式细胞仪检测其外周血CD3、CD4、CD8值，并与正常儿童进行对照，同时检测HSP患儿血清补体系列（C3、C4、CH50）、免疫复合物（CIC）和免疫球蛋白（IgG、IgA、IgM、IgE）。结果HSP患儿CD4及CD4／CD8值明显低于正常儿童（P均〈0．05），而HSP各临床亚型患儿的CD3、CD4、CD8、CD4／CD8、C3、C4、CH50、CIC、IgG、IgA、IgM、IgE等水平差异无显著性。结论T细胞免疫功能紊乱与HSP发病可能存在某种联系。

Study on the T cell subsets in children with Henoch-Schonlein purpura

Objectives To investigate the relationship between T cell subsets and the different subtype of Henoch-Schonlein purpura (HSP),explore the role of T cell subsets in HSP. And further clarify the hypothesis of pathogenesis in different subtype of HSP by assessing the levels of T cell subsets,the complement system,immune complexes and immuno-globulin in patients with HSP. Methods The levels of T cell subsets (CD3?CD4?CD8?CD4/CD8 ) in peripheral blood were determined with flow cytometer in 32 children with HSP in the initial stage without administration of steroid or immune inhibitors. The levels of the complement system(C3?C4?CH50),immune complexes and immuno-globulin(IgG?IgA?IgM?IgE)were also detected. Results The levels of CD3?CD4?and the ratio of CD4/CD8 in children with HSP were significantly lower than that of the controls( P < 0.05),while the difference among subtypes of HSP was not significant. Conclusions The disorder of T cell subsets might be associated with childhood HSP.