高效液相色谱法测定盐酸法舒地尔的含量

目的探讨高效液相色谱法（HPLC）测定盐酸法舒地尔含量的可行性。方法采用高效液相色谱法测定自制盐酸法舒地尔含量。色谱柱：phecda C18（250 mm×4.6 mm,5μm）,以1.0%三乙胺水溶液（用磷酸调节pH值至7.0）-甲醇（50∶50）为流动相;检测波长为275 nm,流速：1.0 ml/min;进样量：20μl;柱温：25℃。结果 HPLC法测定盐酸法舒地尔的浓度在0.048 4~0.242 0 g/L的范围内,浓度与峰面积有良好的线性关系（r=0.9994,n=5）,平均回收率为（99.4±0.47）%,RSD为0.47%（n=9）。结论 HPLC法测定简单,专属性强、灵敏度高,可以用于盐酸法舒地尔的含量测定。

Determination of fasudil hydrochloride content by high performance liquid chromatography

Objective To investigate the feasibility of determination of fasudil hydrochloride content by high performance liquid chromatography（ HPLC）. Methods HPLC was used to determine the content of a self-prepared fasudil hydrochloride solution.Separation was performed on the phecda C18 column（ 250 mm × 4. 6 mm,5 μm） with the mobile phase of triethylamine solution（ the pH value adjusted to 7. 0 using phosphoric acid）- methanol（ 50∶ 50）,at a detection wave length of 275 nm,a flow rate of 1. 0 ml /min,feed volume of 20 μl and column temperature of 25 ℃. Results With HPLC,the content level was within 0. 0484- 0. 2420 g /L.Linear relationship could clearly be noted between the content and the peak area（ r = 0. 9994,n = 5）. Average recovery rate was（ 99. 4 ±0. 47） % and relative standard deviation（ RSD） was 0. 47%（ n = 9）. Conclusion HPLC was a simple,sensitive and specific detection method,which could be used for the determination of fasudil hydrochloride content.